NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE152451 Query DataSets for GSE152451
Status Public on Dec 21, 2020
Title Intravenous injection of oncolytic virus M1 awakes antitumor T cells and overcomes resistance to checkpoint blockade
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Gene set enrichment analysis (GSEA) revealed that upon M1 treatment, apoptotic and inflammation associated genes were largely upregulated, suggesting that M1 killed tumor cells and triggered inflammatory response. The GSEA also revealed that DNA repair pathway was down-regulated in M1 treated tumors, which is consistent with our previous finding that M1 induces DNA damage and then leads to tumor cells apoptosis. Additionally, gene sets marked the release of immune-boosting cytokines such as interferon (IFN)-α, IFN-γand tumor necrosis factor (TNF)-α were deployed in M1 treated tumors, providing potential pro-inflammatory signals to generate antitumor immunity. All the results above demonstrate that M1 induces ICD and shapes TME to an inflammatory state.
 
Overall design C57BL/6J mice were implanted subcutaneously in the right flank with B16F10 cells at day 0 and treated intravenously with PBS (n=4) or C6V1(M1) (n=4) (1x107 pfu) daily on day 6-10. Tumors were harvested on day 12, total RNA was extracted and then performed RNA-sequencing.
 
Contributor(s) Liu Y, CLiu X
Citation(s) 33311488
Submission date Jun 15, 2020
Last update date Dec 21, 2020
Contact name liu xincheng
E-mail(s) chaoyanglxc123@gmail.com
Organization name sysu
Street address zhongshan Road 74 Street
City Guangzhou
ZIP/Postal code 5100000
Country China
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (8)
GSM4616048 C6V1_26
GSM4616049 C6V1_63
GSM4616050 C6V1_72
Relations
BioProject PRJNA639448
SRA SRP267314

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152451_expr.xlsx 1.1 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap