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| Status |
Public on Jun 18, 2020 |
| Title |
Expression profiling by RNA-Seq of 3T3 mouse pre-adipocyte cells after treatment with Indoxyl Sulphate or Oxidative LDL, with or without pNaKtide |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed expression profiling by RNA-Seq on 3T3 mouse pre-adipocyte cells. Cells were divided into six groups of five replicates each and were treated with either control (DMSO), Indoxyl Sulphate, or Oxidative LDL, either with or without pNaKtide (NaK).
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| Overall design |
RNA was extracted from cells in each group, and sequenced in a 2x50 base-pair design on an Illumina 1500 sequencer.
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| Contributor(s) |
Sodhi K, Fan J, Denvir J, Liu J, Juan S, Yiliang C, Roy S, Xie Z, Abraham NG, Shapiro JI |
| Citation(s) |
32824688 |
| Submission date |
Jun 17, 2020 |
| Last update date |
Sep 08, 2020 |
| Contact name |
James Denvir |
| E-mail(s) |
denvir@marshall.edu
|
| Organization name |
Marshall University School of Medicine
|
| Department |
Biochemistry and Microbiology
|
| Lab |
Genomics and Bioinformatics Core Facility
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| Street address |
One John Marshall Drive
|
| City |
Huntington |
| State/province |
WV |
| ZIP/Postal code |
25755 |
| Country |
USA |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE152725 |
Oxidant Alterations in Adipocyte mRNA Expression: Role of the Na,K-ATPase Oxidant Amplification Loop |
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| Relations |
| BioProject |
PRJNA640220 |
| SRA |
SRP267762 |
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