NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE15281 Query DataSets for GSE15281
Status Public on Dec 31, 2009
Title Gene-level expression data in melanocytes and in melanoma cell lines WM1552C and A375 over-expressing miR211
Organism Homo sapiens
Experiment type Expression profiling by array
Summary MicroRNAs (miRNAs) influence cancer development through post-transcriptional negative regulation of both tumor suppressors and oncogenes. We subjected melanoma cell lines, normal melanocytes, and keratinocytes to array based miRNA profiling, and identified several distinct miRNAs with differential expression. Specifically, miR-211 levels were depleted in all eight melanoma cell lines examined, and also in 23 of 30 distinct patient melanoma samples (graded as primary in situ, regional metastatic, distant metastatic and nodal metastatic). Putative target genes of miR-211 were identified, and their anticipated increased expression levels were confirmed in melanoma cell lines, which were reduced in two melanoma cell lines that artificially over-expressed miR-211. Four such target genes (TCF12, RAB22A, KCNMA1 and SLC37A3) were confirmed by a target cleavage assay. Stable over-expression of miR-211 in two melanoma cell lines caused significant growth inhibition and reduced invasiveness. The differential expression of miR-211 in a variety of melanoma cell lines and clinical samples, consistent inverse correlation between miR-211 and its target mRNA levels, and growth retardation and reduced invasiveness of melanoma cell lines by miR-211 are all consistent with the idea that the depletion of miR-211 is a key step in melanoma development and/or progression
 
Overall design The 15 Samples in this submission represent gene-level expression profiling of isolated total RNA from WM1552C, WM1552+miRNA211, A375, A375+miRNA211 and melanocytes hybridized to Affymetrix exon ararys.
 
Contributor(s) Meister E, Perera R
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 18, 2009
Last update date Feb 18, 2019
Contact name ranjan joseph perera
E-mail(s) pererra1@memorialhealth.com
Phone 9123500415
Fax 9123501281
Organization name Anderson Cancer Center
Department DLOR
Lab Oncology
Street address 4700 Waters Ave
City Savannah
State/province GA
ZIP/Postal code 31411
Country USA
 
Platforms (1)
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (15)
GSM381584 Melanocytes_R_1
GSM381585 Melanocytes_R_2
GSM381586 Melanocytes_R_3
Relations
BioProject PRJNA116507

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15281_RAW.tar 340.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap