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| Status |
Public on Jun 20, 2020 |
| Title |
Gene expression profiles in microglia with or without PGC-1α overexpression after acute ischemic stroke (AIS) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
PGC-1α overexpression in microglia protects against ischemia-induced brain damage in mice. To investigate the underlying mechanism of PGC-1α, we have employed whole mRNA microarray expression profiling as a discovery platform to identify genes with the potential to change the microglial function. Indeed, PGC-1α overexpression alters the gene expression profiles of microglia after AIS, this suggested that microglial PGC-1α might play an important role after ischemic stroke.
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| Overall design |
Primary microglia were isolated from PGC-1αf/f and mPGC-1α mice with AIS using the MojoSortTM mouse P2RY12 selection kit (Biolegend, San Diego, CA, USA) according to the manufacturer’s protocol. Then the samples were processed to perform microarray analysis.
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| Contributor(s) |
Han B, Hao J |
| Citation(s) |
33771213 |
| Submission date |
Jun 19, 2020 |
| Last update date |
Apr 05, 2021 |
| Contact name |
Bin Han |
| E-mail(s) |
hanbin1128@163.com
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| Organization name |
Tianjin Medical University General Hospital
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| Street address |
154 Anshan Road, Heping District
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| City |
Tianjin |
| ZIP/Postal code |
300052 |
| Country |
China |
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| Platforms (1) |
| GPL21163 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA640674 |
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