 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 12, 2021 |
| Title |
Changes in gene expression after mechanical loading with and without satellite cell. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Skeletal muscle is a plastic tissue, adapting to different stimuli. Mechanical overload causes muscle hypertrophy and substantial changes in gene expression. We perform a time course analysis following synergistic ablation surgery to induce compensatory hypertrophy in the plantaris muscle in satellite cell depleted mice and wild-type mice. Microarray was used to determine the different expressed genes in skeletal muscle with or without satellite cell following mechanical overload.
|
| |
|
| Overall design |
Mice were treated with tamoxifen to induce satellite cell (SC) depletion or vehicle (control). Following SC ablation, SC depleted mice (SC-) and satellite cell competent mice (SC+, or wild type) were subjected to mechanical overload of the plantaris muscle induced by synergist ablation surgery (removal of soleus and gastrocnemius tendon). Each experimental group had samples collected after 1, 3, 5 and 7 days post synergistic ablation in addition to a sham surgery control. Total RNA was isolated from n of 5-6 for each group per time point. For microarray analysis, samples were pooled with a total of n=2 per time point.
|
| |
|
| Contributor(s) |
Figueiredo VC, Vechetti IJ, Mccarthy JJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 30, 2020 |
| Last update date |
Jul 13, 2021 |
| Contact name |
Vandre C Figueiredo |
| E-mail(s) |
v.casagrandefigueiredo@uky.edu
|
| Organization name |
University of Kentucky
|
| Lab |
Vandre C Figueiredo
|
| Street address |
Rose street
|
| City |
Lexington |
| State/province |
Kentucky |
| ZIP/Postal code |
40502 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (20)
|
| GSM4646859 |
Tam treated plantaris muscle, 1D overloaded, rep1 |
| GSM4646860 |
Tam treated plantaris muscle, 1D overloaded, rep2 |
| GSM4646861 |
Tam treated plantaris muscle, 3D overloaded, rep1 |
| GSM4646862 |
Tam treated plantaris muscle, 3D overloaded, rep2 |
| GSM4646863 |
Tam treated plantaris muscle, 5D overloaded, rep1 |
| GSM4646864 |
Tam treated plantaris muscle, 5D overloaded, rep2 |
| GSM4646865 |
Tam treated plantaris muscle, 7D overloaded, rep1 |
| GSM4646866 |
Tam treated plantaris muscle, 7D overloaded, rep2 |
| GSM4646867 |
Tam treated plantaris muscle, sham operated, rep1 |
| GSM4646868 |
Tam treated plantaris muscle, sham operated, rep2 |
| GSM4646869 |
Veh treated plantaris muscle, 1D overloaded, rep1 |
| GSM4646870 |
Veh treated plantaris muscle, 1D overloaded, rep2 |
| GSM4646871 |
Veh treated plantaris muscle, 3D overloaded, rep1 |
| GSM4646872 |
Veh treated plantaris muscle, 3D overloaded, rep2 |
| GSM4646873 |
Veh treated plantaris muscle, 5D overloaded, rep1 |
| GSM4646874 |
Veh treated plantaris muscle, 5D overloaded, rep2 |
| GSM4646875 |
Veh treated plantaris muscle, 7D overloaded, rep1 |
| GSM4646876 |
Veh treated plantaris muscle, 7D overloaded, rep2 |
| GSM4646877 |
Veh treated plantaris muscle, sham operated, rep1 |
| GSM4646878 |
Veh treated plantaris muscle, sham operated, rep2 |
|
| Relations |
| BioProject |
PRJNA745472 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE153542_RAW.tar |
21.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...