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| Status |
Public on Jul 20, 2020 |
| Title |
scRNAseq of activated cells of the B cell lineage in the tumor microenvironment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goals of this study were to gain a better understanding of the heterogeneity and transcriptional profile of activated cells of the B cell lineage in the tumor microenvironment.
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| |
|
| Overall design |
Activated cells of the B cell lineage (live CD3/14/16-CD19+IgD-CD71+) from primary tumors and metastatic lymph nodes of 3 HPV+ HNSCC patients and, as comparison, from PBMC of a healthy individual 7 days post seasonal Influenza vaccination were FACS-purified. Single-cell suspensions of FACS-purified cells were loaded onto the 10X Genomics Chromium Controller. Library construction was performed using the Chromium Single Cell 5’ Library Construction Kit followed by sequencing on a HiSeq3000 or NovaSeq6000.
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| |
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| Contributor(s) |
Wieland A, Ahmed R |
| Citation(s) |
33208941 |
| Submission date |
Jun 30, 2020 |
| Last update date |
Sep 15, 2021 |
| Contact name |
William Hudson |
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (7)
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| Relations |
| BioProject |
PRJNA643236 |
| SRA |
SRP269383 |
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