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Series GSE15359 Query DataSets for GSE15359
Status Public on Feb 20, 2010
Title Gene Expression Profiling of Airway and Distal Lung Fibroblasts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Fresh lung tissue specimens from distal lungs (n=3) and airways (n=3) were obtained through bronchoscopy, and primary fibroblasts were isolated and cultured. IRB approval was obtained from the University of Pittsburgh Institutional Review Board.
 
Overall design Total RNA was isolated from lung tissue using Qiazol (QIAGEN) and purified using the miRNeasy Mini kit (QIAGEN). RNA quantity and quality was assessed using Agilent Nanodrop and Agilent 2100 Bioanalyzer, respectively. 500 ng of total RNA meeting was Cy3 labeled using the Agilent Low RNA Input Linear Amplification Kit PLUS (One-Color). After purification and fragmentation, aliquots of each sample were hybridized to an Agilent Whole Human Genome 4 X 44K microarray, sequentially washed, and scanned on an Agilent DNA microarray scanner.
 
Contributor(s) Konishi K, Zhou X, Milosevic J, Kaminski N, Wenzel S
Citation(s) 21757679
Submission date Mar 23, 2009
Last update date Feb 22, 2018
Contact name Kazuhisa Konishi
E-mail(s) konishi.kazuhisa@med.osaka-cu.ac.jp
Phone 412-647-3156
Organization name University of Pittsburgh
Department Pulmonry, Allergy, and Critical Care Medicine
Lab Kaminski Lab
Street address 1212 Kaufmann Building 3471 Fifth Ave
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (6)
GSM385592 0349Q_EBBX airway fibroblasts rep1
GSM385593 0944E_EBBX airway fibroblasts rep2
GSM385594 1257Q_EBBX airway fibroblasts rep3
Relations
BioProject PRJNA115779

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15359_RAW.tar 53.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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