|
Status |
Public on Feb 20, 2010 |
Title |
Gene Expression Profiling of Airway and Distal Lung Fibroblasts |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
Fresh lung tissue specimens from distal lungs (n=3) and airways (n=3) were obtained through bronchoscopy, and primary fibroblasts were isolated and cultured. IRB approval was obtained from the University of Pittsburgh Institutional Review Board.
|
|
|
Overall design |
Total RNA was isolated from lung tissue using Qiazol (QIAGEN) and purified using the miRNeasy Mini kit (QIAGEN). RNA quantity and quality was assessed using Agilent Nanodrop and Agilent 2100 Bioanalyzer, respectively. 500 ng of total RNA meeting was Cy3 labeled using the Agilent Low RNA Input Linear Amplification Kit PLUS (One-Color). After purification and fragmentation, aliquots of each sample were hybridized to an Agilent Whole Human Genome 4 X 44K microarray, sequentially washed, and scanned on an Agilent DNA microarray scanner.
|
|
|
Contributor(s) |
Konishi K, Zhou X, Milosevic J, Kaminski N, Wenzel S |
Citation(s) |
21757679 |
Submission date |
Mar 23, 2009 |
Last update date |
Feb 22, 2018 |
Contact name |
Kazuhisa Konishi |
E-mail(s) |
konishi.kazuhisa@med.osaka-cu.ac.jp
|
Phone |
412-647-3156
|
Organization name |
University of Pittsburgh
|
Department |
Pulmonry, Allergy, and Critical Care Medicine
|
Lab |
Kaminski Lab
|
Street address |
1212 Kaufmann Building 3471 Fifth Ave
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15213 |
Country |
USA |
|
|
Platforms (1) |
GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
Samples (6)
|
GSM385595 |
0349Q_TBBX distal lung fibroblasts rep1 |
GSM385596 |
0944E_TBBX distal lung fibroblasts rep2 |
GSM385597 |
1257Q_TBBX distal lung fibroblasts rep3 |
|
Relations |
BioProject |
PRJNA115779 |