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Series GSE15389 Query DataSets for GSE15389
Status Public on Mar 26, 2009
Title Peripheral blood mononuclear cells treated with a CDK inhibitor
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A genomics-based approach to identify pharmacodynamic biomarkers was used for a CDK (cyclin-dependent kinase) inhibitory drug. R547 is a potent CDK inhibitor with a potent anti-proliferative effect at pharmacologically relevant doses, and is currently in Phase I clinical trials. Utilizing preclinical data derived from microarray experiments, we identified pharmacodynamic biomarkers to test in blood samples from patients in clinical trials. These candidate biomarkers were chosen based on several criteria: relevance to the mechanism of action of R547, dose responsiveness in preclinical models, and measurable expression in blood samples. We identified 26 potential biomarkers of R547 action and tested their clinical validity in patient blood samples by quantitative real-time PCR analysis.
 
Overall design 40 samples were initially run, 37 passed QC and were analyzed, samples were run in 6 independent replicates and were treated with either vehicle, R547 at the IC90, or R547 at 3 times the IC90. Additionally, untreated time zero samples were analyzed as a further control
 
Contributor(s) Berkofsky-Fessler W, Rosinski J
Citation(s) 19755512
Submission date Mar 25, 2009
Last update date Mar 25, 2019
Contact name James Rosinski
E-mail(s) jim.rosinski@gmail.com
Organization name Roche
Department In Silico Sciences
Street address 340 Kingsland St
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (37)
GSM386225 PBMCs treated at 3xIC90 for 24 hours,replicate 1
GSM386226 PBMCs treated at 3xIC90 for 24 hours,replicate 2
GSM386227 PBMCs treated at 3xIC90 for 24 hours,replicate 3
This SubSeries is part of SuperSeries:
GSE15396 Peripheral blood mononuclear, DU145, and HCT116 cells treated with a CDK inhibitor
Relations
BioProject PRJNA122945

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15389_RAW.tar 169.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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