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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 26, 2009 |
| Title |
Peripheral blood mononuclear cells treated with a CDK inhibitor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A genomics-based approach to identify pharmacodynamic biomarkers was used for a CDK (cyclin-dependent kinase) inhibitory drug. R547 is a potent CDK inhibitor with a potent anti-proliferative effect at pharmacologically relevant doses, and is currently in Phase I clinical trials. Utilizing preclinical data derived from microarray experiments, we identified pharmacodynamic biomarkers to test in blood samples from patients in clinical trials. These candidate biomarkers were chosen based on several criteria: relevance to the mechanism of action of R547, dose responsiveness in preclinical models, and measurable expression in blood samples. We identified 26 potential biomarkers of R547 action and tested their clinical validity in patient blood samples by quantitative real-time PCR analysis.
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| Overall design |
40 samples were initially run, 37 passed QC and were analyzed, samples were run in 6 independent replicates and were treated with either vehicle, R547 at the IC90, or R547 at 3 times the IC90. Additionally, untreated time zero samples were analyzed as a further control
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| Contributor(s) |
Berkofsky-Fessler W, Rosinski J |
| Citation(s) |
19755512 |
| Submission date |
Mar 25, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
James Rosinski |
| E-mail(s) |
jim.rosinski@gmail.com
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| Organization name |
Roche
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| Department |
In Silico Sciences
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| Street address |
340 Kingsland St
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| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (37)
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| GSM386225 |
PBMCs treated at 3xIC90 for 24 hours,replicate 1 |
| GSM386226 |
PBMCs treated at 3xIC90 for 24 hours,replicate 2 |
| GSM386227 |
PBMCs treated at 3xIC90 for 24 hours,replicate 3 |
| GSM386228 |
PBMCs treated at 3xIC90 for 24 hours,replicate 5 |
| GSM386229 |
PBMCs treated at 3xIC90 for 24 hours,replicate 6 |
| GSM386230 |
PBMCs treated at 3xIC90 for 2 hours,replicate 2 |
| GSM386231 |
PBMCs treated at 3xIC90 for 2 hours,replicate 3 |
| GSM386232 |
PBMCs treated at 3xIC90 for 2 hours,replicate 4 |
| GSM386233 |
PBMCs treated at 3xIC90 for 2 hours,replicate 5 |
| GSM386234 |
PBMCs treated at 3xIC90 for 2 hours,replicate 6 |
| GSM386235 |
PBMCs treated at the IC90 for 24 hours,replicate 1 |
| GSM386236 |
PBMCs treated at the IC90 for 24 hours,replicate 2 |
| GSM386237 |
PBMCs treated at the IC90 for 24 hours,replicate 3 |
| GSM386238 |
PBMCs treated at the IC90 for 24 hours,replicate 4 |
| GSM386239 |
PBMCs treated at the IC90 for 24 hours,replicate 5 |
| GSM386240 |
PBMCs treated at the IC90 for 24 hours,replicate 6 |
| GSM386241 |
PBMCs treated at the IC90 for 2 hours,replicate 1 |
| GSM386242 |
PBMCs treated at the IC90 for 2 hours,replicate 2 |
| GSM386243 |
PBMCs treated at the IC90 for 2 hours,replicate 3 |
| GSM386244 |
PBMCs treated at the IC90 for 2 hours,replicate 4 |
| GSM386245 |
PBMCs treated at the IC90 for 2 hours,replicate 5 |
| GSM386246 |
PBMCs treated at the IC90 for 2 hours,replicate 6 |
| GSM386247 |
Untreated PBMCs, replicate 1 |
| GSM386248 |
Untreated PBMCs, replicate 2 |
| GSM386249 |
Untreated PBMCs, replicate 3 |
| GSM386250 |
PBMCs vehicle treated for 24 hours,replicate 1 |
| GSM386251 |
PBMCs vehicle treated for 24 hours,replicate 2 |
| GSM386252 |
PBMCs vehicle treated for 24 hours,replicate 3 |
| GSM386253 |
PBMCs vehicle treated for 24 hours,replicate 4 |
| GSM386254 |
PBMCs vehicle treated for 24 hours,replicate 5 |
| GSM386255 |
PBMCs vehicle treated for 24 hours,replicate 6 |
| GSM386256 |
PBMCs vehicle treated for 2 hours,replicate 1 |
| GSM386257 |
PBMCs vehicle treated for 2 hours,replicate 2 |
| GSM386258 |
PBMCs vehicle treated for 2 hours,replicate 3 |
| GSM386259 |
PBMCs vehicle treated for 2 hours,replicate 4 |
| GSM386260 |
PBMCs vehicle treated for 2 hours,replicate 5 |
| GSM386261 |
PBMCs vehicle treated for 2 hours,replicate 6 |
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| This SubSeries is part of SuperSeries: |
| GSE15396 |
Peripheral blood mononuclear, DU145, and HCT116 cells treated with a CDK inhibitor |
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| Relations |
| BioProject |
PRJNA122945 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15389_RAW.tar |
169.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
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