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Series GSE15392 Query DataSets for GSE15392
Status Public on Mar 26, 2009
Title DU145 tumor cells treated with a CDK inhibitor
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A genomics-based approach to identify pharmacodynamic biomarkers was used for a CDK (cyclin-dependent kinase) inhibitory drug. R547 is a potent CDK inhibitor with a potent anti-proliferative effect at pharmacologically relevant doses, and is currently in Phase I clinical trials. Utilizing preclinical data derived from microarray experiments, we identified pharmacodynamic biomarkers to test in blood samples from patients in clinical trials. These candidate biomarkers were chosen based on several criteria: relevance to the mechanism of action of R547, dose responsiveness in preclinical models, and measurable expression in blood samples. We identified 26 potential biomarkers of R547 action and tested their clinical validity in patient blood samples by quantitative real-time PCR analysis.
Overall design 52 samples were initially run, 45 passed QC and were analyzed, samples were run in 4 independent replicates and were treated with either vehicle, R547 at the IC50 concentration, R547 at the IC90, or R547 at 3 times the IC90. Samples were harvested at 2, 6, and 24 hours post treatment. Additionally, untreated time zero samples were analyzed as a further control
Contributor(s) Berkofsky-Fessler W, Rosinski J
Citation(s) 19755512
Submission date Mar 25, 2009
Last update date Mar 25, 2019
Contact name James Rosinski
Organization name Roche
Department In Silico Sciences
Street address 340 Kingsland St
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (45)
GSM386288 DU145 cells treated at 3xIC90 for 24 hours, replicate c
GSM386289 DU145 cells treated at 3xIC90 for 24 hours, replicate d
GSM386290 DU145 cells treated at 3xIC90 for 2 hours, replicate b
This SubSeries is part of SuperSeries:
GSE15396 Peripheral blood mononuclear, DU145, and HCT116 cells treated with a CDK inhibitor
BioProject PRJNA122947

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Supplementary file Size Download File type/resource
GSE15392_RAW.tar 211.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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