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Series GSE15407 Query DataSets for GSE15407
Status Public on Dec 31, 2009
Title The Nucleus Accumbens Shell and Central Nucleus of the Amygdala of Alcohol-Preferring Rats Following Binge-Drinking
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The objective of this study was to determine changes in gene expression within the extended amygdala following binge-like drinking by alcohol-preferring (P) rats. Adult male P rats were given 1-hr access to 15 and 30% ethanol (EtOH) three times daily for 8 weeks. Rats (n = 10/time point for EtOH and n = 6/time point for water) were killed by decapitation 1, 6 and 24 hr after the last drinking episode. Brains were extracted and rapidly frozen in isopentane in dry ice. RNA was prepared from individual micropunch samples of the nucleus accumbens shell (ACB-sh) and central nucleus of the amygada (CeA); microarray analyses were conducted with Affymetrix Rat 230.2 chips. EtOH intakes were 1.5-2 g/kg/session. Because too few genes changed at the individual time points, an overall effect, comparing the water and EtOH groups, was determined. In the ACB-sh and CeA, there were 276 and 402 probe sets for named genes, respectively, that were different between the two groups. There were 1.5- to 3.5- fold more genes up-regulated than down-regulated in both regions, with most differences between 1.1- to 1.2-fold. Although there were several significant Biological Processes categories in common between the 2 regions (e.g., synaptic transmission, neurite development), there were few genes in common between the two regions that differed between the EtOH and water groups. Overall, the results suggest that chronic binge-like alcohol drinking by P rats produces changes in the expression of genes that could alter neuronal function by different mechanisms in the ACB-sh and CeA.
 
Overall design 17 samples of control and 31 samples of treatment for each brain region
 
Contributor(s) Kimpel MW, Schultz JA, Bell RL, McClintick JN, Edenberg HJ, McBride WJ
Citation(s) 20116196
Submission date Mar 26, 2009
Last update date Jul 31, 2017
Contact name Mark William Kimpel
E-mail(s) mkimpel@iupui.edu
Organization name Indiana University
Department Psychiatry
Lab W.J. McBride
Street address 15032 Hunter Court
City Westfield
State/province IN
ZIP/Postal code 46074
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (96)
GSM386668 Central Nucleus of Amygdala, time from last drinking episode (hrs):1, ethanol-1
GSM386669 Central Nucleus of Amygdala, time from last drinking episode (hrs):1, ethanol-2
GSM386670 Central Nucleus of Amygdala, time from last drinking episode (hrs):6, ethanol-3
Relations
BioProject PRJNA117079

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15407_RAW.tar 281.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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