NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE154279 Query DataSets for GSE154279
Status Public on Jul 13, 2020
Title Nuclear Role for Human Argonaute-1 as an Estrogen-dependent Transcription Coactivator
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In mammals, argonaute (AGO) proteins have been characterized for their roles in small RNA-mediated post-transcriptional and also in transcriptional gene silencing. We report here a different role for AGO1 in estradiol-triggered transcriptional activation in human cells. We show that in MCF-7 mammary gland cells AGO1 associates to transcriptional enhancers of estrogen receptor alpha (ERα) and that this association is upregulated by treating the cells with estrogen (E2), displaying a positive correlation with the activation of these enhancers.
 
Overall design Examination of AGO1 binding to chromatin in vehicle or estradiol treated MCF7 cells
 
Contributor(s) Gómez Acuña LI, Nazer E, Rodríguez-Seguí SA, Pozzi B, Buggiano V, Marasco LE, Agirre E, He C, Alló M, Kornblihtt AR
Citation(s) 32673398
Submission date Jul 12, 2020
Last update date Oct 12, 2020
Contact name Alberto R Kornblihtt
E-mail(s) ark@fbmc.fcen.uba.ar
Organization name CONICET_Universidad de Buenos Aires
Street address Pabellón IFIBYNE, Ciudad Universitaria
City Ciudad Autónoma de Buenos Aires
ZIP/Postal code 1428
Country Argentina
 
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (8)
GSM4668322 AGO1 ChIP MCF7 vehicle Rep1
GSM4668323 AGO1 ChIP MCF7 vehicle Rep2
GSM4668324 AGO1 ChIP MCF7 estradiol Rep1
Relations
BioProject PRJNA645722
SRA SRP271494

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154279_RAW.tar 3.3 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap