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| Status |
Public on Dec 01, 2021 |
| Title |
Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes [1] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Nicotinamide, the amide form of vitamin B3, is essential to maintain the human fetal development. It benefits the ectoderm and endoderm development, but its influence in mesoderm differentiation is elusive. In this study, we reported that nicotinamide regulated the gene expression of cardiovascular system, and it induced functional cardiomyocytes which was independent of canonical WNT signaling. Through a kinase screening, we found that nicotinamide inhibited the activity of P38δ to promote cardiomyocyte differentiation. Furthermore, we compared the gene expression of cardiomyocytes induced by WNT inhibitor IWP-2, nicotinamide or P38 inhibitor SB202190. The results showed that the cardiomyocytes induced by nicotinamide had similar gene expression profile compared with P38 inhibitor, which indicated nicotinamide promoted cardiac differentiation via the inhibition of P38.
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| Overall design |
Total RNA obtained from human embryonic stem cell (hESC) culture in E8 medium for 3 days(E8 72 hrs), E8 medium with nicotinamide 10 mM treatment for 3 days (Nam 72 hrs).
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| Contributor(s) |
Chen G |
| Citation(s) |
34845199 |
| Submission date |
Jul 15, 2020 |
| Last update date |
Dec 10, 2021 |
| Contact name |
Ren Zhili |
| E-mail(s) |
yb57622@umac.mo
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| Organization name |
University of Macau
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| Street address |
Avenida da Universidade
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| City |
Macau |
| ZIP/Postal code |
999078 |
| Country |
China |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE154455 |
Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes |
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| Relations |
| BioProject |
PRJNA646371 |
