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| Status |
Public on Jul 17, 2020 |
| Title |
Mechanism suppressing transcription of the globin genes in Danio rerio nonerythroid cells |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In genomes of modern fish and amphibia α- and β- globin genes are grouped at a single locus that may resemble the ancestral one and is syntenic to α-globin locus of modern warm-blooded vertebrates. In Danio rerio, the major locus of α/β globin genes comprises two subclusters, one of them harboring genes expressed in adult and the other – genes expressed in embryonic and larval erythrocytes. The results of our previous study suggested that the adult subcluster of this locus has evolved into α-globin gene domain of vertebrate animals. Here we studied how adult and embryo-larval genes of Danio rerio major globin gene locus are repressed in fibroblasts. The results obtained suggest that that at least some of the globin genes present within the adult subcluster are repressed by Polycomb similarly to human α-globin genes. Furthermore, within two α/β gene pairs repression of α-type and β-type genes appears to be mediated by different mechanisms as increasing the level of histone acetylation can activate transcription of only β-type genes.
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| Overall design |
Target-enriched ChIP-Seq for H3K27me3, H3panac and H4panac in Danio rerio fibroblasts with and without TSA treatment (the libraries are enriched with the sequences of a/b-globin gene locus and flanking areas).
Each sample in this series represents two replicates. The raw data for the replicates are represented as two independent SRR.
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| Contributor(s) |
Iarovaia O |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 16, 2020 |
| Last update date |
Oct 27, 2020 |
| Contact name |
Natalia Klimenko |
| E-mail(s) |
lklimenko@genebiology.ru
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| Phone |
9150884603
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| Organization name |
Institute of Gene Biology (IGB) of the Russian Academy of Sciences
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| Street address |
34/5 Vavilova Street
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| City |
Moscow |
| ZIP/Postal code |
143026 |
| Country |
Russia |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA646701 |
| SRA |
SRP272232 |
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