|
| Status |
Public on Jul 01, 2021 |
| Title |
Long-term treatment with senolytic drugs Dasatinib and Quercetin ameliorates age-dependent intervertebral disc degeneration in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Intervertebral disc degeneration is highly prevalent in the elderly population and is a leading cause of chronic back pain and disability. Studies have linked degenration with increased disc cell senescence. Likewise, senolytics such as Dasatinib + Quercetin combination may provide a novel approach to mitigate age-dependnt disc degeneration.
We used microarrays to explore the transcriptomics of differentially expressed genes between aged disc compartments: AF vs NP at 23M, AF: D+Q vs Veh and NP: D+Q vs Veh.
|
| |
|
| Overall design |
NP and AF tissue were dissected from lumbar discs from BL6 mice Vehicle (n=5) and D+Q treated mice (n=4). Mice were treated weekly with intraperitoneal injections of either Vehicle (1:2 PBS and 1:2 DMSO) or Dasatinib, 5 mg/kg (Sigma-Aldrich, CDS023389) plus Quercetin, 50 mg/kg (Sigma-Aldrich, Q4951) until they became 23-months-old. RNA extraction and hybridization on Affymetrix microarrays.
disc compartment, treatment
|
| |
|
| Contributor(s) |
Novais EJ |
| Citation(s) |
34480023 |
| Submission date |
Jul 17, 2020 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Emanuel José Novais |
| E-mail(s) |
emanuel.j.novais@gmail.com
|
| Organization name |
Thomas Jefferson
|
| Department |
Department of Orthopaedic Surgery
|
| Street address |
1025 Walnut St., Suite 511 College Bldg.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (18)
|
| GSM4675944 |
01_CT4N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675945 |
02_CT5N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675946 |
03_CT6N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675947 |
04_CT7N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675948 |
05_CT15N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675949 |
06_DQ8N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675950 |
07_DQ11N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675951 |
08_DQ16N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675952 |
09_DQ17N_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675953 |
10_CT4A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675954 |
11_CT5A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675955 |
12_CT6A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675956 |
13_CT7A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675957 |
14_CT15A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675958 |
15_DQ8A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675959 |
16_DQ11A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675960 |
17_DQ16A_Emanual_9-12-19_(Clariom_S_Mouse) |
| GSM4675961 |
18_DQ17A_Emanual_9-12-19_(Clariom_S_Mouse) |
|
| Relations |
| BioProject |
PRJNA646873 |
Less...
More...