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| Status |
Public on Aug 20, 2009 |
| Title |
The regulation of reserve carbohydrate metabolism in S cerevisiae in response to nutrient availability |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
In Saccharomyces cerevisiae, glycogen and trehalose are important reserve carbohydrates that accumulate under nutrient limitation in batch cultures. An inherent draw-back of batch studies is that specific growth rate and substrate and product concentrations are variable over time and between cultures. The aim of this present study was to identify the nutritional requirements associated with high accumulation of reserve carbohydrates at a fixed specific growth rate (0.10 h-1) in anaerobic chemostat cultures that were limited by one of five different nutrients (carbon, nitrogen, sulfur, phosphorus or zinc). Reserve carbohydrates accumulation is not a general response to nutrient limitation. Over the conditions tested, accumulation occurs essentially under nitrogen (and to a lesser extent carbon) limited conditions. This was confirmed by the transcriptional profile of the genes involved in trehalose biosynthesis. We show that the transcriptional induction of both glycogen and trehalose biosynthesis genes was to a large extent driven by the regulator Msn2/4. However, the main regulatory control of glycogen biosynthesis was post-translational. Under nitrogen limitation, the ratio of glycogen synthase over glycogen phosphorylase increased up to eight-fold, thus enabling an increased flux towards glycogen biosynthesis.
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| Overall design |
We studied this in anaerobic chemostat cultures at a dilution rate of 0.10 h-1 where growth was limited by five different nutrients (carbon, nitrogen, sulfur, phosphorus or zinc limitations). In addition, we studied the expression of these pathways at transcriptional and post-transcriptional levels and assessed the role of Msn2/4 in mediating transcriptional induction of glycogen and trehalose genes in the absence of stress.
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| Contributor(s) |
Hazelwood LA, Pronk JT, Daran J |
| Citation(s) |
19734328 |
| Submission date |
Mar 31, 2009 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Jean-Marc Daran |
| E-mail(s) |
j.g.daran@tudelft.nl
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| Phone |
+31 15 278 2412
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| Organization name |
Delft University of Technology
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| Department |
Department of Biotechnology
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| Lab |
Kluyver centre for genomics of industrial organisms
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| Street address |
Julianalaan 67
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| City |
Delft |
| ZIP/Postal code |
2628BC |
| Country |
Netherlands |
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| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
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| Samples (21)
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| GSM137497 |
C-lim Anaerobic reference (pH 5) #1 |
| GSM137498 |
C-lim Anaerobic reference (pH 5) #2 |
| GSM137675 |
C-lim Anaerobic reference (pH 5) #3 |
| GSM198367 |
Zinc-limited Anaerobic chemostat culture-1 |
| GSM198368 |
Zinc-limited Anaerobic chemostat culture-2 |
| GSM198369 |
Zinc-limited Anaerobic chemostat culture-3 |
| GSM200691 |
Nitorgen-limited Anaerobic chemostat culture-1 |
| GSM200692 |
Nitrogen-limited Anaerobic chemostat culture-3 |
| GSM200693 |
Nitrogen-limited Anaerobic chemostat culture-2 |
| GSM387533 |
S-lim anaerobic chemostat, dilution rate 0.1 h-1 -1 |
| GSM387534 |
S-lim anaerobic chemostat, dilution rate 0.1 h-1 -2 |
| GSM387535 |
S-lim anaerobic chemostat, dilution rate 0.1 h-1 -3 |
| GSM387536 |
P-lim anaerobic chemostat, dilution rate 0.1 h-1 -1 |
| GSM387537 |
P-lim anaerobic chemostat, dilution rate 0.1 h-1 -2 |
| GSM387538 |
P-lim anaerobic chemostat, dilution rate 0.1 h-1 -3 |
| GSM387539 |
C-lim anaerobic chemostat of msn2msn4 double mutant -1 |
| GSM387540 |
C-lim anaerobic chemostat of msn2msn4 double mutant -2 |
| GSM387541 |
C-lim anaerobic chemostat of msn2msn4 double mutant -3 |
| GSM387542 |
N-lim anaerobic chemostat of msn2msn4 double mutant -1 |
| GSM387543 |
N-lim anaerobic chemostat of msn2msn4 double mutant -2 |
| GSM387544 |
N-lim anaerobic chemostat of msn2msn4 double mutant -3 |
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| Relations |
| BioProject |
PRJNA115903 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15465_RAW.tar |
57.1 Mb |
(http)(custom) |
TAR (of CEL, CHP, EXP) |
| Processed data included within Sample table |
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