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| Status |
Public on May 19, 2021 |
| Title |
RNA-sequencing from miR-375 knockout and YAP overexpression lung carcinoid (H727) cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report RNA sequencing data for miR-375 knockout and YAP overexpression lung carcinoid cells (H727). Lung carcinoids are variably aggressive and mechanistically understudied neuroendocrine neoplasms (NENs). Here, we identified and elucidated the function of a miR-375/yes-associated protein (YAP) axis in lung carcinoid (H727) cells. miR-375 and YAP are respectively high and low expressed in wild-type H727 cells. Following lentiviral CRISPR/Cas9-mediated miR-375 depletion, we identified distinct transcriptomic changes including dramatic YAP upregulation. Similarly, YAP overexpression resulted in distinct and partially overlapping transcriptomic changes, phenocopying the effects of miR-375 depletion in the same models as above. Pathways analysis and confirmatory real-time PCR studies of shared dysregulated targets indicate that this axis controls neuroendocrine related functions such as neural differentiation, exocytosis, and secretion. Taken together, we provide compelling evidence that a miR-375/YAP axis is a critical mediator of neuroendocrine differentiation and tumorigenesis in lung carcinoid cells.
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| |
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| Overall design |
Examination of transcriptional changes resulting from miR-375 knockout and YAP overexpression in lung carcinoid cells
|
| Web link |
https://0-www-nature-com.brum.beds.ac.uk/articles/s41598-021-89855-4#Sec25
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| |
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| Contributor(s) |
Yang X, Nanayakkara J, Renwick N |
| Citation(s) |
34001972 |
| Submission date |
Jul 21, 2020 |
| Last update date |
May 19, 2021 |
| Contact name |
Neil Renwick |
| E-mail(s) |
neil.renwick@queensu.ca
|
| Organization name |
Queen's University
|
| Street address |
88 Stuart Street
|
| City |
Kingston |
| State/province |
ON |
| ZIP/Postal code |
K7L3N6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (12)
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|
| Relations |
| BioProject |
PRJNA647731 |
| SRA |
SRP273175 |
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