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Series GSE155126 Query DataSets for GSE155126
Status Public on May 05, 2021
Title mRNA Expression Changes in Organotin-Driven NLRP3 Inflammasome Activation in Murine Bone Marrow Derived Macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Trimethyltin (TMT) and triethyltin (TET) are two tri-organotin compounds, a group of substances reported to affect mitochondria function. These compounds were also found to activate the NLRP3 inflammasome, an inflammatory cascade that occurs in myeloid cells when primed with a Toll-like receptor ligand (i.e. LPS) followed by activation by an exogenous or endogenous danger signal. The inflammasome is a very tightly regulated mechanism, with several miRNAs playing an important role. To determine differential miRNA changes between TMT and TET induced inflammasome activation, miRNA expression profiles were evaluated in LPS-primed murine bone marrow derived macrophages (BMDMs) exposed to TMT or TET as compared to saline. mRNA expression arrays were also performed and analyzed to identify miRNA target genes in order to elucidate the key mechanism(s) by which TMT and TET activate the NLRP3 inflammasome pathway. These studies will help us understand the inflammasome regulation in macrophages by organotin.
Overall design Murine bone marrow derived macrophages were exposed to LPS (33ng/ml) as a priming agent for 3 hours followed by either TMT OH (1.25 microMolar), TET (10 microMolar), or saline to induce NLRP3 inflammasome activation. Comparisons were performed between each organotin and saline. Three biological replicates for each group were set up.
Contributor(s) Harry GJ, Childers G, Sieber S, Li JL
Citation(s) 33929904
Submission date Jul 26, 2020
Last update date May 05, 2021
Contact name Jian-Liang Li
Organization name NIEHS
Street address 111 TW Alexander Dr
City Durham
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM4695867 LPS Replicate Replicate 1
GSM4695868 LPS Replicate Replicate 2
GSM4695869 LPS Replicate Replicate 3
This SubSeries is part of SuperSeries:
GSE155128 mRNA Expression and microRNA Expression Changes in Organotin-Driven NLRP3 Inflammasome Activation in Murine Bone Marrow Derived Macrophages
BioProject PRJNA648753

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155126_RAW.tar 33.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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