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| Status |
Public on Jul 13, 2010 |
| Title |
Genome-wide analysis of gene expression perturbed by reptin shRNA in MCF7 subjected to normoxic and hypoxic conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to investigate the role of reptin methylation on the expression of hypoxia-responsive genes across the whole genome, we performed a microarray analysis from RNAs isolated from MCF7 cells expressing either control shRNA (shRNA) or reptin shRNA (shreptin) in normoxic and hypoxic conditions.
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| Overall design |
MCF7 cells were transiently transfected with either non-specific shRNA or reptin shRNA in replicates. Cells were grown for 24 hours before being exposed to either normoxic (21% oxygen) or hypoxic (1% oxygen) conditions for 18 hours. Cells were then lysed and RNA was isolated.
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| Contributor(s) |
Baek SH, Hwang D, Lee JS, Kim Y, Kim IS, Noh SC, Kim M |
| Citation(s) |
20603076 |
| Submission date |
Apr 02, 2009 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Minhyung Kim |
| E-mail(s) |
Minhyung.Kim@cshs.org
|
| Organization name |
Cedars-Sinai Medical Center
|
| Department |
Urology
|
| Street address |
8700 Beverly Blvd
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA115709 |
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