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| Status |
Public on Apr 01, 2010 |
| Title |
Expression profiling of HeLa cells at S and G2/M phases of mitotic cell cycle. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We compared the mRNAs expression profile of HeLa cells between two phases of the mitotic cell cycle: S and G2/M phases. Results provide insight into the regulation of transcript levels during mitotic cell cycle progression. HeLa cells were synchronized with double thymidine blockade (12 hours with 2 mM thymidine, 12 hours release, and 12 hours with 2 mM thymidine), and cells were taken after 2 hours release (S phase) and 8 hours release (G2/M phase).
Keywords: time course
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| Overall design |
Comparison of mRNAs measured at S phase (2 hours release after double thymidine blockade) and at G2/M phase (8 hours release after double thymidine blockade); 3 biological replicates at each of the two time points; two technical replicates with dye swapping per comparison; additional comparison between G2/M (8h) and S (2h).
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| |
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| Contributor(s) |
Novoa I, Gallego J, Mendez R |
| Citation(s) |
20364142 |
| Submission date |
Apr 03, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Isabel Novoa |
| E-mail(s) |
isabel.novoa@crg.es
|
| Organization name |
Center for Genomic Regulation
|
| Department |
Gene Regulation
|
| Street address |
Dr. Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL887 |
Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version) |
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| Samples (6)
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| GSM389382 |
S phase versus G2/M phase replicate 1 |
| GSM389383 |
S phase versus G2/M phase replicate 2 |
| GSM389384 |
S phase versus G2/M phase replicate 3 |
| GSM389385 |
S phase versus G2/M phase replicate 1 dye-swap |
| GSM389386 |
S phase versus G2/M phase replicate 2 dye-swap |
| GSM389387 |
S phase versus G2/M phase replicate 3 dye-swap |
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| Relations |
| BioProject |
PRJNA115985 |
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