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Series GSE15561 Query DataSets for GSE15561
Status Public on Jan 23, 2010
Title Generation of parthenogenetic iPS cells from parthenogenetic neural stem cell
Organism Mus musculus
Experiment type Expression profiling by array
Summary In pluripotential reprogramming, a pluripotent state is established within somatic cells. In this study, we have generated induced pluripotent stem (iPS) cells from bi-maternal (uniparental) parthenogenetic neural stem cells (pNSCs) by transduction with four (Oct4, Klf4, Sox2, and c-Myc) or two (Oct4 and Klf4) transcription factors. The parthenogenetic iPS (piPS) cells directly reprogrammed from pNSCs were able to generate germline-competent himeras, and hierarchical clustering analysis showed that piPS cells were clustered more closer to parthenogenetic ES cells than normal female ES cells. Interestingly, piPS cells showed loss of parthenogenetic-specific imprinting patterns of donor cells. Microarray data also showed that the maternally imprinted genes, which were not expressed in pNSCs, were upregulated in piPS cells, indicating that pluripotential reprogramming lead to induce loss of imprinting as well as re-establishment of various features of pluripotent cells in parthenogenetic somatic cells.
 
Overall design 5 samples were analyzed by microarray, each one them in duplicate.
fNSC: Mouse female NSC (Neural Stem Cell)
pNSC: Mouse parthenogenetic NSC (Neural Stem Cell)
piPS-2F: Mouse parthenogenetic induced pluripotent cells derived from NSC overexpressing Oct4 and Klf4
pESC-B: Mouse parthenogenetic ESC (Embryonic Stem Cell) SSEA-1 sorted
fESC: Mouse female ESC (Embryonic Stem Cell) OG2
 
Contributor(s) Do JT, Joo JY, Han DW, Araúzo-Bravo MJ, Kim MJ, Greber B, Zaehres H, Sobek-Klocke I, Chung HM, Schöler HR
Citation(s) 19816953
Submission date Apr 06, 2009
Last update date Jun 14, 2018
Contact name Marcos J Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (10)
GSM389514 fNSC rep1
GSM389515 fNSC rep2
GSM389516 pNSC rep1
Relations
BioProject PRJNA115653

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15561_Non-NormalizedData.txt 5.0 Mb (ftp)(http) TXT
GSE15561_RAW.tar 3.1 Mb (http)(custom) TAR
Raw data are available on Series record
Processed data included within Sample table

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