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Series GSE15568 Query DataSets for GSE15568
Status Public on Nov 28, 2013
Title Gene expression in rectal epithelia of cystic fibrosis patients
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Gene expression profiles were recorded from rectal suction specimens of Cystic Fibrosis (CF) patients, carrying the CF-specific D508 mutated CFTR-allele. These profiles were compared with gene expression profiles from rectal suction specimens of non-CF subjects (control).
Overall design We used Affymetrix GeneChip HG-U133A microarrays to record the gene expression profile of 16 CF and 13 non-CF individuals.
Rectal suction specimens representing rectal mucosal epithelia were collected from CF patients and non-CF individuals. Biopsies were analyzed for residual function using ICM and total RNA was isolation immediately using the Qiagen RNeasy protocol with on column DNA digestion. About 1-5 µg RNA were obtained from each individual. Integrity of total RNA samples was monitored by gel-electrophoresis before cDNA synthesis. Labeled cRNA synthesis and hybridization was performed following standard protocols.
Contributor(s) Wölfl S, Pusch L, Kroll TC, van Barneveld A, Stanke F, Tamm S, Siebert B, Ballmann M, Junge S, Tümmler B
Citation(s) 24105369, 21731057
Submission date Apr 06, 2009
Last update date Oct 31, 2019
Contact name Stefan Wolfl
Organization name University Heidelberg
Department IPMB, Biology
Lab Bioanalytik
Street address Im Neuenheimer Feld 364
City Heidelberg
ZIP/Postal code D-69120
Country Germany
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (29)
GSM389652 CF (D508) subject1
GSM389653 CF (D508) subject2
GSM389654 CF (D508) subject3
BioProject PRJNA115739

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15568_RAW.tar 104.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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