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| Status |
Public on Aug 31, 2009 |
| Title |
Expression data from Drosophila Kc167 cell line |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
The consitutive activation of the JAK/STAT signalling cascade is reponsible for the majority of meyoproliferative disorders in humans, a disease that is also conserved in Drosophila. A gain-of-function mutation in the Drosophila JAK kinase leads to blood cell (haemocyte) overproliferation which eventually is manifested as black melanotic tumors. Haemocyte-like Drosophila Kc167 cells were used to identify downstream target genes of the JAK/STAT pathway which may be responsible for tumour generation and progression.
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| Overall design |
Kc167 cells were activated with the principal JAK/STAT pathway ligand Unpaired (UPD) in a time course manner. To this end, for each transcript profiling condition and time point, biological duplicates were activated with UPD or Mock conditioned media for 30 min, and total RNA was extracted 2, 4 or 10h after initial addition of conditioned media. 12 samples were used for hybridization to the arrays.
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| Contributor(s) |
Bina S, Zeidler M |
| Citation(s) |
20168330 |
| Submission date |
Apr 07, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Martin Zeidler |
| Organization name |
University of Sheffield
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| Department |
Dept of Biomedical Science
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| Street address |
Western Bank
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| City |
Sheffield |
| ZIP/Postal code |
S102TN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA115591 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15584_RAW.tar |
22.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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