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Status |
Public on Mar 11, 2021 |
Title |
The translational landscape of phenylephrine-treated neonatal rat cardiomyocytes [ssRNA-seq] |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In this study, we generated nucleotide-resolution translatome as well as transcriptome data of isolated primary cardiomyocytes undergoing hypertrophy. More than 10,000 open reading frames (ORF) were detected from the deep sequencing of ribosome protected fragments orchestrating the shift of translatome in hypertrophied cardiomyocytes.We further identified more than 100 potential micropeptides encoded by uncharacterized small open reading frames (sORFs) in the long noncoding RNA genes. Over two-thirds of these micropeptide candidates were experimentally validated in a random test with three micropeptides showing regulatory function in cardiomyocyte hypertrophy via modulating the activities of Oxidative phosphorylation, Calcium signaling pathway, and MAPK signaling pathway.Our study provided a genome-wide overview of the translational controls of cardiomyocyte hypertrophy and demonstrated an unrecognized role of micropeptides in cardiomyocyte biology.
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Overall design |
Ribo-seq and RNA-seq of neonatal rat cardiomyocytes treated with or without PE; RNA-seq of neonatal rat cardiomyocytes infected with ad-sORF or control virus ssRNA-seq data presented here.
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Contributor(s) |
Yan Y, Tang R, Li B, Cheng L, Yang J, Huang Z |
Citation(s) |
33677093 |
Submission date |
Aug 08, 2020 |
Last update date |
Jun 10, 2021 |
Contact name |
Youchen Yan |
E-mail(s) |
yanych8@mail2.sysu.edu.cn
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Organization name |
The First Affiliated Hospital, Sun Yat-sen University
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Street address |
No.58 Zhongshan Road II
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City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510080 |
Country |
China |
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Platforms (1) |
GPL24688 |
HiSeq X Ten (Rattus norvegicus) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE155914 |
The translational landscape of phenylephrine-treated neonatal rat cardiomyocytes |
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Relations |
BioProject |
PRJNA713923 |