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Series GSE155913 Query DataSets for GSE155913
Status Public on Mar 11, 2021
Title The translational landscape of phenylephrine-treated neonatal rat cardiomyocytes [ssRNA-seq]
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary In this study, we generated nucleotide-resolution translatome as well as transcriptome data of isolated primary cardiomyocytes undergoing hypertrophy. More than 10,000 open reading frames (ORF) were detected from the deep sequencing of ribosome protected fragments orchestrating the shift of translatome in hypertrophied cardiomyocytes.We further identified more than 100 potential micropeptides encoded by uncharacterized small open reading frames (sORFs) in the long noncoding RNA genes. Over two-thirds of these micropeptide candidates were experimentally validated in a random test with three micropeptides showing regulatory function in cardiomyocyte hypertrophy via modulating the activities of Oxidative phosphorylation, Calcium signaling pathway, and MAPK signaling pathway.Our study provided a genome-wide overview of the translational controls of cardiomyocyte hypertrophy and demonstrated an unrecognized role of micropeptides in cardiomyocyte biology.
 
Overall design Ribo-seq and RNA-seq of neonatal rat cardiomyocytes treated with or without PE; RNA-seq of neonatal rat cardiomyocytes infected with ad-sORF or control virus
ssRNA-seq data presented here.
 
Contributor(s) Yan Y, Tang R, Li B, Cheng L, Yang J, Huang Z
Citation(s) 33677093
Submission date Aug 08, 2020
Last update date Jun 10, 2021
Contact name Youchen Yan
E-mail(s) yanych8@mail2.sysu.edu.cn
Organization name The First Affiliated Hospital, Sun Yat-sen University
Street address No.58 Zhongshan Road II
City Guangzhou
State/province Guangdong
ZIP/Postal code 510080
Country China
 
Platforms (1)
GPL24688 HiSeq X Ten (Rattus norvegicus)
Samples (4)
GSM4715670 NRVCs treated with PE_ssRNA_seq_rep1
GSM4715671 NRVCs treated with PE_ssRNA_seq_rep2
GSM4715672 NRVCs control_ssRNA_seq_rep1
This SubSeries is part of SuperSeries:
GSE155914 The translational landscape of phenylephrine-treated neonatal rat cardiomyocytes
Relations
BioProject PRJNA713923

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155913_CPE_DESeq2_results_2rep_annotation.txt.gz 1.3 Mb (ftp)(http) TXT
GSE155913_CPE_counts.txt.gz 277.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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