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Series GSE15601 Query DataSets for GSE15601
Status Public on Jun 15, 2009
Title Uncovering the Arabidopsis thaliana nectary transcriptome
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Many flowering plants attract pollinators by offering a reward of floral nectar. Remarkably, the molecular events involved in the development of nectaries, the organs that produce nectar, as well as the synthesis and secretion of nectar itself, are poorly understood. Indeed, to date, no genes have been shown to directly affect the de novo production or quality of floral nectar. To address this gap in knowledge, the ATH1 Affymetrix GeneChip array was used to systematically investigate the Arabidopsis nectary transcriptome to identify genes and pathways potentially involved in nectar production. In this study, we identified a large number of genes differentially expressed between secretory lateral nectaries and non-secretory median nectary tissues, as well as between mature lateral nectaries (post-anthessis) and immature lateral nectary tissue (pre-anthesis).
 
Overall design Three different types of RNA samples were prepared from Arabidopsis thaliana ecotype Columbia-0 nectaries: mature lateral nectaries (MLN; Stage 14-15 flowers), immature lateral nectaries (ILN, Stage 11-12 flowers), and mature median nectaries (MMN, Stage 14-15 flowers) [developmental stages defined by (Smyth et al., 1990)]. MLN are secretory tissues, whereas, ILN and MMN are pre-secretory and nonsecretory tissues, respectively. All nectary tissues were separately dissected by hand from the flowers of primary inflorescences of ca. 30-35 day-old plants. All plants were grown in soil with a 16h light/8h dark light regimen. Due to the small size of nectaries, dissections took place over several days from 4-8 hours after dawn (h.a.d.).
 
Contributor(s) Kram BW, Xu WW, Carter CJ
Citation(s) 19604393
Submission date Apr 08, 2009
Last update date Aug 28, 2018
Contact name Clay J Carter
E-mail(s) cjcarter@d.umn.edu
Phone 218-726-7347
Organization name University of Minnesota Duluth
Department Biology
Street address 1035 Kirby Dr
City Duluth
State/province MN
ZIP/Postal code 55812
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (8)
GSM390161 lateral nectary, stage 14-15 rep1
GSM390162 lateral nectary, stage 14-15 rep2
GSM390163 lateral nectary, stage 14-15 rep3
Relations
BioProject PRJNA115723

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15601_RAW.tar 27.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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