|
| Status |
Public on Jun 07, 2021 |
| Title |
GATA6 defines endoderm fate by controlling chromatin accessibility during differentiation of human induced pluripotent stem cells [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Investigation of the role played by GATA6 in establishing the definitive endoderm chromatin accessbility profile. We used pluripotent stem cells as a model of early development. We derived GATA6-/- pluripotent cells with an inducible GATA6 construct that permits exongenous GATA6 cDNA expression upon supplmentation of doxycycline. We differentiated GATA6 +/+ and GATA6-/- (with and without doxycyline) cells to definitive endoderm and analyzed the gene expression profile by RNA-seq.
|
| |
|
| Overall design |
Examination of gene expression during definitive endoderm formation in the absence and presence of GATA6.
|
| |
|
| Contributor(s) |
Heslop JA, Duncan SA |
| Citation(s) |
34010638 |
| Submission date |
Aug 11, 2020 |
| Last update date |
Jun 07, 2021 |
| Contact name |
Stephen A Duncan |
| E-mail(s) |
duncanst@musc.edu
|
| Phone |
843-792-9104
|
| Organization name |
Medical University South Carolina
|
| Department |
Regenerative Medicine and Cell Biology
|
| Lab |
Duncan Lab
|
| Street address |
173 Ashley Avenue, BSB 6th Floor, Room 657A
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (18)
|
| GSM4720545 |
RNA - Day 2 - R2 - GATA6+/+ |
| GSM4720546 |
RNA - Day 2 - R1 - GATA6Ex4∆1/∆2;ind GATA6 (-dox) |
| GSM4720547 |
RNA - Day 2 - R2 - GATA6Ex4∆1/∆2;ind GATA6 (-dox) |
| GSM4720548 |
RNA - Day 2 - R1 - GATA6Ex4∆1/∆2;ind GATA6 (+dox) |
| GSM4720549 |
RNA - Day 2 - R2 - GATA6Ex4∆1/∆2;ind GATA6 (+dox) |
| GSM4720550 |
RNA - Day 4 - R1 - GATA6+/+ |
| GSM4720551 |
RNA - Day 4 - R2 - GATA6+/+ |
| GSM4720552 |
RNA - Day 4 - R1 - GATA6Ex4∆1/∆2;ind GATA6 (-dox) |
| GSM4720553 |
RNA - Day 4 - R2 - GATA6Ex4∆1/∆2;ind GATA6 (-dox) |
| GSM4720554 |
RNA - Day 4 - R1 - GATA6Ex4∆1/∆2;ind GATA6 (+dox) |
| GSM4720555 |
RNA - Day 4 - R2 - GATA6Ex4∆1/∆2;ind GATA6 (+dox) |
| GSM4720556 |
RNA - Day 6 - R1 - GATA6+/+ |
| GSM4720557 |
RNA - Day 6 - R2 - GATA6+/+ |
| GSM4720558 |
RNA - Day 8 - R1 - GATA6+/+ |
| GSM4720559 |
RNA - Day 8 - R2 - GATA6+/+ |
|
| This SubSeries is part of SuperSeries: |
| GSE156021 |
GATA6 defines endoderm fate by controlling chromatin accessibility during differentiation of human induced pluripotent stem cells. |
|
| Relations |
| BioProject |
PRJNA656447 |
| SRA |
SRP277025 |
Less...
More...