|
| Status |
Public on Aug 16, 2021 |
| Title |
Genome-wide profiling of histone H1 variants and H3K9me3 in T47D-MTVL cells upon multiple H1 depletion |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Human somatic cells may contain up to seven members of the histone H1 family contributing to chromatin compaction and regulation of nuclear processes, apparently with certain subtype specificities. In breast cancer cells, the combined depletion of H1.2 and H1.4 has a strong deleterious effect, deregulates many genes, promotes the appearance of accessibility sites genome-wide, and triggers an interferon response via activation of heterochromatic repeats. We have further analyzed the consequences of multiple H1 variants depletion by profiling the occupancy of five somatic histone H1 variants and H3K9me3 post-translational modification in T47D-MTVL cancer cells. Specifically, stable breast cancer-derived cell lines expressing an shRNA against multiple histone H1 isoforms in response to doxycycline (Dox) were grown for six days in the presence or absence of Dox. ChIP-Seq experiments were subsequently performed to analyze the genome-wide localization of histone H1 variants and H3K9me3.
|
| |
|
| Overall design |
Genome-wide analysis of H1.0, H1.2, H1.4, H1.5, and H1X linker histones, and H3K9me3 epigenetic modification in T47D-MTVL cells expressing a Dox-inducible shRNA against multiple H1 variants.
|
| |
|
| Contributor(s) |
Jordan A, Serna N, Salinas M |
| Citation(s) |
35380694, 38261975, 38530350 |
|
Núria Serna-Pujol, Mónica Salinas-Pena, Francesca Mugianesi, François Le Dily, Marc A. Marti-Renom, and Albert Jordan. Coordinated changes in gene expression, H1 variant distribution and genome 3D conformation in response to H1 depletion. bioRxiv 2021.02.12.429879. doi:10.1101/2021.02.12.429879
|
| Submission date |
Aug 11, 2020 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Albert Jordan |
| E-mail(s) |
ajvbmc@ibmb.csic.es
|
| Organization name |
IBMB-CSIC
|
| Department |
Molecular Genomics
|
| Street address |
Baldiri Reixac 4
|
| City |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platforms (1) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA656518 |
| SRA |
SRP277052 |
Less...
More...