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| Status |
Public on Nov 25, 2009 |
| Title |
Whole genome transcription profile of antigen receptor activated B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To search for rapid changes in gene expression following BCR activation, we performed DNA microarray analysis of activated splenic B cells with and without anti-IgM treatment for 3 hour. The expression of a remarkably large set of genes differed significantly.
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| Overall design |
Primary B-lymphocytes were purified from mouse spleens and stimulated with IL-4 (Pepro-Tech) and CD40L (R&D Systems) as indicated at 5 ng/ml and 200 ng/ml, respectively. The BCR of stimulated cells was activated by incubation with goat F(ab’)2 anti-mouse IgM (Southern Biotechnologies) at 2.5 μg/ml for 3 hour.
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| |
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| Contributor(s) |
Verma-Gaur J, Grundström T |
| Citation(s) |
19553523 |
| Submission date |
Apr 08, 2009 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Thomas Grundström |
| E-mail(s) |
thomas.grundstrom@molbiol.umu.se
|
| Phone |
+46 90 785 2531
|
| Organization name |
Umeå University
|
| Department |
Department of Molecular Biology
|
| Lab |
TGN Lab
|
| Street address |
Analysvägen 1
|
| City |
Umeå |
| ZIP/Postal code |
901 87 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA115731 |
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