|
| Status |
Public on Mar 25, 2021 |
| Title |
Single T-cell landscape identifies tissue regeneration program in murine and human regulatory T cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Several studies over the past decade identified a subpopulation of murine regulatory T cells (Tregs) which have the intrinsic capacity to promote tissue homeostasis and, upon damage, enhance tissue regeneration and re-organization. In humans, this population of tissue regeneration-promoting Treg cells has not been identified and characterized yet. Using single-cell chromatin accessibility as well as single-cell RNA and TCR expression profiles of murine and human tissue-derived CD4 T cells, we identified the murine Treg repair signature and performed a liftover on human single T cell data. We identified a FOXP3+CCR8+HLA-DR+ population in peripheral blood, which has characteristics of human tissue Treg cells and promotes tissue regeneration and wound healing in-vitro. This population was dependent on the transcriptional regulator BATF, a factor that also drives the development of tissue regeneration-promoting Treg cells in the murine system. Human CCR8+HLA-DR+ Treg cells could be used as a novel T-cell product to promote tissue repair in regenerative medicine.
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| |
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| Overall design |
Isolation of CD4+ T cells and CD25+ T cells from murine tissues and spleen
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| |
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| Contributor(s) |
Michael D, Malte S, Nicholas S, Markus F |
| Citation(s) |
33789089 |
| Submission date |
Aug 12, 2020 |
| Last update date |
Apr 28, 2021 |
| Contact name |
LIT NGS-Core |
| E-mail(s) |
ngs-core@lit.eu
|
| Phone |
+49 941 944-38100
|
| Organization name |
Leibniz Institute for Immunotherapy
|
| Department |
NGS-Core
|
| Street address |
Franz-Josef-Strauß-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
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| Platforms (1) |
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| Samples (17)
|
|
| Relations |
| BioProject |
PRJNA656792 |
| SRA |
SRP277203 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156112_Mouse_Gnotobiotic_CD4_Seurat.RDS.gz |
2.0 Gb |
(ftp)(http) |
RDS |
| GSE156112_Mouse_Gnotobiotic_CD4_barcodes.tsv.gz |
126.2 Kb |
(ftp)(http) |
TSV |
| GSE156112_Mouse_Gnotobiotic_CD4_fragments.tsv.gz |
4.5 Gb |
(ftp)(http) |
TSV |
| GSE156112_Mouse_Gnotobiotic_CD4_matrix.mtx.gz |
510.0 Mb |
(ftp)(http) |
MTX |
| GSE156112_Mouse_Gnotobiotic_CD4_peaks.bed.gz |
626.6 Kb |
(ftp)(http) |
BED |
| GSE156112_Mouse_Gnotobiotic_CD4_singlecell.csv.gz |
11.9 Mb |
(ftp)(http) |
CSV |
| GSE156112_Mouse_Normal_CD4_Seurat.RDS.gz |
2.9 Gb |
(ftp)(http) |
RDS |
| GSE156112_Mouse_Normal_CD4_barcodes.tsv.gz |
127.2 Kb |
(ftp)(http) |
TSV |
| GSE156112_Mouse_Normal_CD4_fragments.tsv.gz |
9.7 Gb |
(ftp)(http) |
TSV |
| GSE156112_Mouse_Normal_CD4_matrix.mtx.gz |
859.6 Mb |
(ftp)(http) |
MTX |
| GSE156112_Mouse_Normal_CD4_peaks.bed.gz |
981.2 Kb |
(ftp)(http) |
BED |
| GSE156112_Mouse_Normal_CD4_singlecell.csv.gz |
20.8 Mb |
(ftp)(http) |
CSV |
| GSE156112_Mouse_Tumor_CD4_Seurat.RDS.gz |
370.5 Mb |
(ftp)(http) |
RDS |
| GSE156112_Mouse_Tumor_CD4_barcodes.tsv.gz |
44.4 Kb |
(ftp)(http) |
TSV |
| GSE156112_Mouse_Tumor_CD4_fragments.tsv.gz |
1.4 Gb |
(ftp)(http) |
TSV |
| GSE156112_Mouse_Tumor_CD4_matrix.mtx.gz |
101.1 Mb |
(ftp)(http) |
MTX |
| GSE156112_Mouse_Tumor_CD4_peaks.bed.gz |
490.9 Kb |
(ftp)(http) |
BED |
| GSE156112_Mouse_Tumor_CD4_singlecell.csv.gz |
5.1 Mb |
(ftp)(http) |
CSV |
| GSE156112_RAW.tar |
17.1 Gb |
(http)(custom) |
TAR (of BED, CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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