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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 28, 2009 |
| Title |
Expression data from mNSc after 48 hour of treatment with CD95L-T4 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In neural stem cells, stimulation of the “death receptor” CD95 does not trigger apoptosis but resulted in increased stem cell survival and neuronal specification via activation of the Src /PI3K /AKT/mTOR signalling pathway. To further characterize CD95-dependent neural stem cell survival and differentiation we used conventional gene expression profiling combined with translation state array analysis. Mouse neural stem cells grown in neurosphere cultures were stimulated with a trimerized CD95L construct (CD95L-T4) and total as well as polysomal bound RNA was isolated 48 hours after stimulation and analysed by microarrays. CD95L-T4 treatment induced a global increase in ribosome-bound mRNA and protein translation as well as changes on genes involved in neurogenesis, protein synthesis and transcription factors.
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| Overall design |
Mouse neural stem cells grown in neurosphere cultures were stimulated with a trimerized CD95L construct (CD95L-T4) and total as well as polysomal bound RNA was isolated 48 hours after stimulation and hybridized on affymetrix microarrays.
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| Contributor(s) |
Corsini N, Martin-Villalba A, Gretz N, Kumar S |
| Citation(s) |
19664992 |
| Submission date |
Apr 10, 2009 |
| Last update date |
May 04, 2018 |
| Contact name |
sachin kumar |
| E-mail(s) |
s.kumar@dkfz.de
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| Organization name |
DKFZ
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| Street address |
Molecular Neurobiology; German Cancer Research Center; INF 581;
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| City |
Heidelberg |
| State/province |
Baden-Württemberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (15)
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| GSM391199 |
mNSC Treated with CD95L-T4 at total rna level, biological rep1 |
| GSM391200 |
mNSC Treated with CD95L-T4 at total rna level, biological rep2 |
| GSM391201 |
mNSC Treated with CD95L-T4 at total rna level, biological rep3 |
| GSM391202 |
mNSC Treated with CD95L-T4 at total rna level, biological rep4 |
| GSM391203 |
mNSC control at total rna level, biological rep1 |
| GSM391204 |
mNSC control at total rna level, biological rep2 |
| GSM391205 |
mNSC control at total rna level, biological rep3 |
| GSM391206 |
mNSC control at total rna level, biological rep4 |
| GSM391207 |
mNSC Treated with CD95L-T4 at polysomal rna level, biological rep1 |
| GSM391208 |
mNSC Treated with CD95L-T4 at polysomal rna level, biological rep2 |
| GSM391209 |
mNSC Treated with CD95L-T4 at polysomal rna level, biological rep3 |
| GSM391210 |
mNSC control at polysomal rna level, biological rep1 |
| GSM391211 |
mNSC control at polysomal rna level, biological rep2 |
| GSM391212 |
mNSC control at polysomal rna level, biological rep3 |
| GSM391213 |
mNSC control at polysomal rna level, biological rep4 |
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| Relations |
| BioProject |
PRJNA116713 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15623_RAW.tar |
49.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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