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| Status |
Public on Mar 31, 2021 |
| Title |
Expression data of peritoneal macrophages from Wild-type mouse cultured in control or serine/glycine-depleted medium. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Activation of signaling pathways downstream of Toll-like receptor 4 upregulate expression of genes related to serine metabolism. We cultured peritoneal macrophages in control or serine/glycine-depleted medium for 24 hr, stimulated them with LPS (10 ng/ml) for 6 or 12 hr, and collected them to compare the gene expression.
Microarray analysis revealed that serine/glycine-depletion in peritoneal macrophages upregulates gene expression of enzymes for serine biosynthesis.
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| Overall design |
We obtained primary peritoneal macrophages from 3% thioglycollate-treated mice 4 days after the injection, cultured them in control or serine/glycine-depleted medium for 24 hr, stimulated them with LPS (10 ng/ml) for 6 or 12 hr, and collected them for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Ito A, Kurita K, Suganami T |
| Citation(s) |
34045514 |
| Submission date |
Aug 17, 2020 |
| Last update date |
Jun 30, 2021 |
| Contact name |
Kento Kurita |
| Phone |
+81-52-789-3883
|
| Organization name |
Nagoya University
|
| Department |
Department of Molecular Medicine and Metabolism, Research Institute of Environmental Medicine
|
| Street address |
Furo-cho
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-0814 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| GSM4729089 |
Peritoneal macrophage Control, Veh 6 hr |
| GSM4729090 |
Peritoneal macrophage Control, LPS 6 hr |
| GSM4729091 |
Peritoneal macrophage Serine/glycine-depleted, Veh 6 hr |
| GSM4729092 |
Peritoneal macrophage Serine/glycine-depleted, LPS 6 hr |
| GSM4729093 |
Peritoneal macrophage Control, Veh 12 hr |
| GSM4729094 |
Peritoneal macrophage Control, LPS 12 hr |
| GSM4729095 |
Peritoneal macrophage Serine/glycine-depleted, Veh 12 hr |
| GSM4729096 |
Peritoneal macrophage Serine/glycine-depleted, LPS 12 hr |
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| Relations |
| BioProject |
PRJNA657535 |
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