|
| Status |
Public on May 19, 2021 |
| Title |
Microarray gene expression in RKO cell line under low replication stress |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
RKO cells were treated with low doses of aphidicolin (0,2µM) that inhibit replicative DNA polymerases and induce a mild replication stress. Expression data were analysed on control cells (DMSO), 16h of treatment (t0) and after release in complete new culture medium of 13h (N+1) using microarray (affymetrix Clarion S)
We used microarray to analyse the impact of low replication stress on gene expression comparing DMSO or aphidicolin-treated cells at the end of the treatment (t0) and in daughter cells released from the replication stress (N+1).
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| |
|
| Overall design |
Triplicates of total RNA was extracted from the same amount of exponentially growing RKO cells in different conditions (DMSO or aphidicolin, t0 or N+1) and hybridized on Affymetrix Microarrays.
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| |
|
| Contributor(s) |
Bergoglio V |
| Citation(s) |
34066960 |
|
https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22094959
|
| Submission date |
Aug 19, 2020 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Valérie Bergoglio |
| E-mail(s) |
valerie.bergoglio@inserm.fr
|
| Phone |
0582741655
|
| Organization name |
INSERM UMR1037
|
| Lab |
CRCT
|
| Street address |
2 avenue Hubert Curien
|
| City |
Toulouse cedex 1 |
| ZIP/Postal code |
31037 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
|
| Samples (12)
|
| GSM4732913 |
RKO Aphidicolin treated for 16h, rep1 |
| GSM4732914 |
RKO Aphidicolin treated for 16h, rep2 |
| GSM4732915 |
RKO Aphidicolin treated for 16h, rep3 |
| GSM4732916 |
RKO DMSO treated and released for 13h, rep1 |
| GSM4732917 |
RKO DMSO treated and released for 13h, rep2 |
| GSM4732918 |
RKO DMSO treated and released for 13h, rep3 |
| GSM4732919 |
RKO Aphidicolin treated for 16h and released for 13h, rep1 |
| GSM4732920 |
RKO Aphidicolin treated for 16h and released for 13h, rep2 |
| GSM4732921 |
RKO Aphidicolin treated for 16h and released for 13h, rep3 |
|
| Relations |
| BioProject |
PRJNA658154 |
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