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Series GSE156727 Query DataSets for GSE156727
Status Public on Sep 02, 2020
Title Primary normal human bronchial epithelial cells (NHBE) cultured at air liquid interface with or without macrophage cells (+/- Mac) derived from primary peripheral blood mononuclear cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose is to obtain samples for mRNA analysis in primary normal human bronchial epithelial cells cultured with or without macrophage cells (+/- Mac) derived from primary peripheral blood monnumclear cells
 
Overall design Influenza A/Vietnam/1203/2004 (H5N1, VN1203) or influenza A/California/04/2009 (H1N1, CA04) viral stocks were used to infect NHBE cells at a multiplicity of infection (MOI) of 1. Infections were allowed to progress for 1 hour. Next, 500,000 peripheral blood mononuclear cell (PBMC)-derived macrophages were place onto each apical transwell surface in a final volume of 500 ul to create co­ cultures. The co-cultures at a ratio of approximately 2 NHBE cells for every PBMC-derived macrophage were allowed to progress for an additional 11, 15 and 23 hours respectively (12, 16, 24 hours post-infection). An n=5 was used for each condition, which included infection with either H1N1, H5N1, or mock uninfected, of either NHBE-only monocultures or NHBE + PBMC-macrophage co-cultures. In total this infection scheme represented a total of 90 samples for microarray analysis. At the specified time points, 1 ml of TRlzol RNA isolation reagent (Thermo Fischer, 15596-016) that lead to inactivation of samples.
 
Contributor(s) Powell J, Waters KM, Heller N, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 24, 2020
Last update date Sep 04, 2020
Contact name Natalie Heller
E-mail(s) natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (86)
GSM4743104 Mock_NM_12hr_1
GSM4743105 Mock_NM_12hr_3
GSM4743106 Mock_NM_12hr_4
Relations
BioProject PRJNA660617

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156727_RAW.tar 179.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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