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Series GSE156870 Query DataSets for GSE156870
Status Public on Aug 26, 2020
Title Targeting steroid receptor co-activator 3 sensitizes myeloma cell to proteasome inhibitor treatment through NSD2-mediated phase separation and chromatin remodeling [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we demonstrate that NSD2/SRC-3 complex coordinates histone H3 lysine 36 dimethylation (H3K36me2) and transcriptional elongation factor Pol II to regulate chromatin dynamic and gene transcription during myeloma resistant to bortezomib. Mechanistically, NSD2 can interact with SRC-3, its SET domain is responsible to H3K36me2 to enhance the transcriptional activity of SRC-3 target gene. The inhibitor of SRC-3, SI-2, can impaired the interaction between NSD2 and SRC-3, caused the distribution of H3K36me2 on the genome-wide.
 
Overall design Examination of histone modification in multiple myeloma bortezomib-resistant cell line
 
Contributor(s) Liu J, Xie Y
Citation(s) 33589584
Submission date Aug 25, 2020
Last update date Mar 02, 2021
Contact name Liu Zhiqiang
E-mail(s) zhiqiangliu@tmu.edu.cn
Organization name Tianjin Medical University
Street address 22 Qxiangtai Rd
City Tianjin
ZIP/Postal code 300070
Country China
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (6)
GSM4745854 MM.1S BR-H3K36me2-IP
GSM4745855 MM.1S BR-SI2- H3K36me2-IP
GSM4745856 MM.1S BR-Pol II-IP
This SubSeries is part of SuperSeries:
GSE156872 Targeting steroid receptor co-activator 3 sensitizes myeloma cell to proteasome inhibitor treatment through NSD2-mediated phase separation and chromatin remodeling
Relations
BioProject PRJNA659375
SRA SRP278913

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156870_RAW.tar 1.3 Gb (http)(custom) TAR (of BW, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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