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| Status |
Public on Apr 18, 2009 |
| Title |
A complementary role for ELF3 and TFL1 in the regulation of flowering time by ambient temperature. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Plants regulate their time to flowering by gathering information from the environment. Photoperiod and temperature are among the most important environmental variables. Suboptimal, but not near-freezing, temperatures regulate flowering through the thermosensory pathway, which overlaps with the autonomous pathway. Here we show that ambient temperature regulates flowering by two genetically distinguishable pathways, one that requires TFL1 and another that requires ELF3. The delay in flowering time observed at lower temperatures was partially suppressed in single elf3 and tfl1 mutants, whereas double elf3 tfl1 mutants were insensitive to temperature. tfl1 mutations abolished the temperature response in cryptochrome mutants that are deficient in photoperiod perception, but not in phyB mutants that have a constitutive photoperiodic response. Contrary to tfl1, elf3 mutations were able to suppress the temperature response in phyB mutants, but not in cryptochrome mutants. The gene expression profile revealed that the tfl1 and elf3 effects are due to the activation of different sets of genes and identified CCA1 and SOC1/AGL20 as being important cross talk points. Finally, genome-wide gene expression analysis strongly suggests a general and complementary role for ELF3 and TFL1 in temperature signalling.
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| Overall design |
Three genotypes, WT (Columbia), elf3-7 and tfl1-1 mutants. Three biological replicates for each condition (genotype X temperature combination). RNA prepared independently for each sample.
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| Contributor(s) |
Strasser B, Alvarez MJ, Califano A, Cerdan PD |
| Citation(s) |
19187043 |
| Submission date |
Apr 15, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Pablo Diego Cerdan |
| URL |
http://www.leloir.org.ar
|
| Organization name |
Fundacion Instituto Leloir
|
| Lab |
Plant Molecular Biology
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| Street address |
Av. Patricias Argentinas 435
|
| City |
Ciudad de Buenos Aires |
| ZIP/Postal code |
1405 |
| Country |
Argentina |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (18)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA116641 |
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