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Series GSE157086 Query DataSets for GSE157086
Status Public on Sep 01, 2021
Title Large-scale genomic alterations identified in cancer patients on the course of cancer recovery with complementary Huaier treatment identified by total RNA- and small non-coding RNA-sequencing.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Tremetes robiniophila Murr (Huaier), has been well known by many literatures as a significant cancer complementary therapy. We have proved that a broad spectrum of anti-cancer effect of Huaier is based on the rescue of the disrupted Hippo signalling pathway, especially through the recovery in transcriptional dysregulation. We have further investigated the systematic molecular mechanism of cancer recovery as clinical research, using a systematic transcriptome analysis using genome-scale mRNA and small non-coding RNA sequencing.
 
Overall design The obtained peripheral blood samples (from cancer patients as well as normal controls) were designated to be used for RNA extraction, construction of whole transcriptome library for sequencing on DNBSeq Platform, followed by whole transcriptome mapping. We have also performed total non-coding small RNAs sequencing on the same DNBSeq Platform, and the length of small RNAs were found to be between 18 to 30 nucleotides. Total 91 samples from 32 cancer patients were sequenced and analysed.
 
Contributor(s)  Tanaka M, Tanaka T, Teng F, Lin H, Xu C, Li N, Luo Z, Wei D, Lu Z
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Submission date Aug 28, 2020
Last update date Sep 03, 2021
Contact name Manami Tanaka
E-mail(s) huaier_bradeioninstitute@hotmail.com
Organization name Bradeion Institute of Medical Sciences
Street address 2-22-9 Hiratsuka, Kanagawa 259-1211, Japan
City Kanagawa
ZIP/Postal code 221-0045
Country Japan
 
Platforms (1)
GPL23227 BGISEQ-500 (Homo sapiens)
Samples (184)
GSM4752548 small RNA KS10_1
GSM4752549 small RNA KS10_2
GSM4752550 small RNA KS10_3
Relations
BioProject PRJNA660034
SRA SRP279237

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE157086_RAW.tar 273.3 Mb (http)(custom) TAR (of FA, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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