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| Status |
Public on Aug 05, 2021 |
| Title |
Identification of alternative splicing changes upon ablation of cytoplasmic SRSF1. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
SRSF1, shuttles between the nucleus and cytoplasm affecting post-splicing processes. However, the physiological significance of this remains unclear. Here, we used genome editing to knock-in a nuclear retention signal (NRS) in Srsf1 to create a mouse model harboring a non-shuttling SRSF1 protein. We then assessed whether alblation of shuttling activities of SRSF1 affects its nuclear functions.
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| Overall design |
Mouse fibroblasts were derived from wild type or homozygous or heterozygous SRSF1-NRS mice. Total RNA was isolated from MEFs and subject to sequencing. Alternative splicing analysis was performed using Suppa2. Three replicates for WT and homozygous MEFs, and 2 replicates for heterozygous MEFs were used.
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| Contributor(s) |
Haward F, Maslon MM, Yeyati PL, Bellora N, Aitken S, von Kreigsheim A, Mill P, Adams IR, Cáceres JF |
| Citation(s) |
34338635 |
| Submission date |
Sep 01, 2020 |
| Last update date |
Aug 05, 2021 |
| Contact name |
Magdalena Maria Maslon |
| E-mail(s) |
magdalena.maslon@igmm.ed.ac.uk
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| Organization name |
University of Edinburgh
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH42XU |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA660733 |
| SRA |
SRP279592 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE157269_MEF_DE_normalised_counts.xlsx |
2.9 Mb |
(ftp)(http) |
XLSX |
| GSE157269_MEF_WT_HOM_splicing.csv.gz |
4.3 Kb |
(ftp)(http) |
CSV |
| GSE157269_MEF_splicing.xlsx |
98.8 Kb |
(ftp)(http) |
XLSX |
| GSE157269_transcript_quantification_MEF_HET_R1.tsv.gz |
1.8 Mb |
(ftp)(http) |
TSV |
| GSE157269_transcript_quantification_MEF_HET_R2.tsv.gz |
1.9 Mb |
(ftp)(http) |
TSV |
| GSE157269_transcript_quantification_MEF_HOM_R1.tsv.gz |
1.9 Mb |
(ftp)(http) |
TSV |
| GSE157269_transcript_quantification_MEF_HOM_R2.tsv.gz |
1.9 Mb |
(ftp)(http) |
TSV |
| GSE157269_transcript_quantification_MEF_HOM_R3.tsv.gz |
1.9 Mb |
(ftp)(http) |
TSV |
| GSE157269_transcript_quantification_MEF_WT_R1.tsv.gz |
1.8 Mb |
(ftp)(http) |
TSV |
| GSE157269_transcript_quantification_MEF_WT_R2.tsv.gz |
1.9 Mb |
(ftp)(http) |
TSV |
| GSE157269_transcript_quantification_MEF_WT_R3.tsv.gz |
1.9 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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