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| Status |
Public on Sep 19, 2020 |
| Title |
Human G-MDSCs are Neutrophils at Distinct Maturation Stages that Promote Tumor Growth and Immune Cell Exclusion in Breast Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Myeloid-derived suppressor cells (MDSC) are known to contribute to immune evasion in cancer. However, the function of the human granulocytic (G)-MDSC subset during tumor progression is largely unknown, and there are no established markers for their identification in human tumor specimens. Using gene expression profiling, mass cytometry and tumor microarrays, we here demonstrate that human G-MDSCs occur as neutrophils at distinct maturation stages, with a disease-specific profile. G-MDSCs derived from patients with metastatic breast cancer (MBC) and malignant melanoma display a unique immature neutrophil profile, that is more similar to healthy donor neutrophils than to G-MDSCs from sepsis patients. Finally, we show that primary G-MDSCs from MBC patients co-transplanted with breast cancer cells, promote tumor growth and affect vessel formation, leading to myeloid immune cell exclusion. Our findings reveal a role for human G-MDSC in tumor progression and have clinical implications also for targeted immunotherapy.
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| Overall design |
Triplicate blood sample cells, per condition, were FACS sorted, RNA was extracted and hybridized to Affymetrix 1.0 ST arrays.
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| Web link |
https://www.life-science-alliance.org/content/3/11/e202000893
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| |
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| Contributor(s) |
Leandersson K, Mehmeti-Ajradini M, Carlsson R |
| Citation(s) |
32958605 |
| Submission date |
Sep 09, 2020 |
| Last update date |
Sep 24, 2020 |
| Contact name |
Robert Carlsson |
| E-mail(s) |
Robert.Carlsson@med.lu.se
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| Organization name |
Lund University
|
| Department |
IKVL
|
| Lab |
Translational neurology
|
| Street address |
Sölvegatan 17
|
| City |
Lund |
| ZIP/Postal code |
22362 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA662593 |
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