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| Status |
Public on Mar 18, 2011 |
| Title |
Gene expression profiling of human gliomas and human glioblastoma cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify signaling pathways that are differentially regulated in human gliomas, a microarray analysis on 30 brain tumor samples (12 primary glioblastomas (GBM), 3 secondary glioblastomas (GBM-2), 8 astrocytomas (Astro) and 7 oligodendrogliomas (Oligo)) and on 5 glioblastoma cell lines (LN018, LN215, LN229, LN319 and BS149) was performed. Normal brain tissue (NB) and normal human astrocytes (NHA) were used as a control. Kinase expression in each tumor was compared to expression in normal brain and expression values from normal human astrocytes were used as an additional control.
Keywords: Kinase expression in each tumor was compared to expression in normal brain and expression values from normal human astrocytes were used as an additional control.
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| Overall design |
Frozen tissue samples of human gliomas and normal brain obtained from the operating room were processed according to the guidelines of the Ethical Committee of the University Hospitals of Basel. Human brain tumor cell lines were derived from human patients. The BS149 cell line was generated at the University of Basel, Switzerland while the “LN” series were a kind gift of Erwin van Meir in Lausanne, Switzerland. Normal human astrocytes (NHAs) were purchased by Cambrex (Walkersville, MD) and cultured according to manufacturer’s recommendations.
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| Contributor(s) |
Morin PJ, Grzmil M, Oakeley EJ, Hemmings BA, Merlo A, Lino MM |
| Citation(s) |
21406405 |
| Submission date |
Apr 24, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Michal Grzmil |
| E-mail(s) |
michal.grzmil@fmi.ch
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| Phone |
+41 61-6978565
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| Fax |
+41-61-6973976
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| Organization name |
Friedrich Miescher Institute for Biomedical Research
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| Department |
Growth control
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| Lab |
3.46
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA116745 |
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