|
| Status |
Public on Apr 28, 2009 |
| Title |
GBM cell lines derived and propagated according to Cambridge protocol |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We screened the gene expression in GBM cell lines cultured under serum-free conditions. We have special interest in genes associated with glial development such as NG2, Olig2 and PDGFRa. This microarray data gave us an idea about the expression of these genes and made the basis for further investigation.
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| Overall design |
Seven GBM cells were derived from 7 different patients under serum-free conditions according to Cambridge protocol as previously described (Fael Al-Mayhani et al, 2009). The RNA was extracted and its quality was assessed. The samples were sent for microarray screening using Illumina platform. The gene was considered as present when the detection value was greater than 0.99.
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| Contributor(s) |
Fael Al-Mayhani TM, Watts C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 27, 2009 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Talal M. Fael Al-Mayhani |
| Phone |
01223-331160
|
| Fax |
01223-331174
|
| Organization name |
University of Cambridge
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| Department |
Clinical Neurosciences
|
| Street address |
Forvie Site, Robinson Way
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0PY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA116823 |
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