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| Status |
Public on Sep 07, 2004 |
| Title |
27_RA_RMA_Jan04_time_course |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This series represents murine dorsal neural tube bisected along the midline with one half from each embryo used for control and the other half treated with 10-6M RA dissolved in ethanol for 6, 12, 24 or 48 h. For 6 h exposures, the explants were cultured overnight on fibronectin coated 35mm dishes (Biocoat, Becton Dickinson Labware, Bedford, MA) in DMEM with 10% horse serum in order to allow for sufficient outgrowth of neural crest cells. The RA was added the following morning; RMA Express 0.2 used to initially normalize data; GeneSpring (Silicon Genetics, Inc.) used for subsequent analysis
Samples were analyzed at 6, 12, 24, and 48 hours.
Keywords = neural crest
Keywords = microarray
Keywords = retinoic acid
Keywords = gene expression
Keywords = cell signaling
Keywords = cell proliferation
Keywords: time-course
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| Contributor(s) |
Colbert MC, Williams SS |
| Citation(s) |
15466718 |
| Submission date |
Jul 23, 2004 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Sarah S. Williams |
| E-mail(s) |
sarah.williams@cchmc.org
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| Phone |
513.636.2055
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| Organization name |
Cincinnati Children's Hopital Medical Center
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| Street address |
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA90739 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE1588_RAW.tar |
74.1 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
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