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Status |
Public on Nov 10, 2020 |
Title |
Transcriptome and epigenomics characteristics of LPS induced CD127 expressing human monocytes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We performed the transcriptomic and epigenetic analysis for both CD127high and CD127low monocytes under LPS stimulation, and RNA-seq and ATAC-seq were performed accordingly for this purpose.
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Overall design |
PBMC were isolated from buffy coat of healthy blood donors by Ficoll gradients, and CD14+ monocytes were isolated from PBMC with anti-CD14 magnetic beads (Miltenyi Biotec). CD14+ monocytes were treated with LPS for 6 h and then were collected for FACS-based sorting of CD127high and CD127low monocytes. Sorted cells were either directly used for ATAC-seq or TRIzol lysed for RNA extraction.
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Contributor(s) |
Zhang B, Zhang Y |
Citation missing |
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Submission date |
Oct 06, 2020 |
Last update date |
Nov 12, 2020 |
Contact name |
Bin Zhang |
E-mail(s) |
vincent42@sina.cn
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Organization name |
Tsinghua University
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Street address |
Zheng Yutong Building room D311
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City |
Beijing |
ZIP/Postal code |
100084 |
Country |
China |
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Platforms (2) |
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Samples (12)
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Relations |
BioProject |
PRJNA667719 |
SRA |
SRP286552 |