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| Status |
Public on Oct 01, 2021 |
| Title |
Spatial transcriptome of Nematostella vectensis apical organ reveals its molecular signature and associated cells |
| Organism |
Nematostella vectensis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The spatial transcriptome data has allowed us to explore the differential gene expression among the oral-aboral domains and provided new insights into the apical organ signalling pathways for future mechanistic studies and genes associated with oral/aboral differentiation. This resource identifies numerous candidates for elucidating the functional role of the apical organ and to address the evolution of the metazoan larval nervous system.
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| Overall design |
Here, we uncovered the molecular signature of the apical organ in N. vectensis through spatial transcriptomics. We performed microdissection on N. vectensis larvae and carefully separated the aboral tissue from the rest of the larval body at the planula and late planula development stages.We acquired transcriptome data from both the aboral tissue and the rest of the body separately to perform differential gene expression (DGE) analysis.
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| Contributor(s) |
Gilbert E, Teeling C, Modepalli V |
| Citation(s) |
36000354 |
| Submission date |
Oct 07, 2020 |
| Last update date |
Nov 02, 2022 |
| Contact name |
vengamanaidu modepalli |
| E-mail(s) |
vengamanaidumodepalli@gmail.com
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| Organization name |
Marine Biological Association of the UK
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| Department |
Cell and Molecular
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| Lab |
Dr Vengamanaidu Modepalli
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| Street address |
The Laboratory,Citadel Hill
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| City |
Plymouth, Devon |
| State/province |
--- |
| ZIP/Postal code |
PL1 2PB, |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL29226 |
Illumina NovaSeq 6000 (Nematostella vectensis) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA667916 |
| SRA |
SRP286651 |
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