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| Status |
Public on Oct 21, 2020 |
| Title |
Defining the Functional Targets of Cap’n’collar Transcription Factors NRF1, NRF2, and NRF3. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The NRF transcription factors NRF1, NRF2, and NRF3, are a subset of Cap’n’collar transcriptional regulators which modulate the expression of genes harboring antioxidant-response element (ARE) sequences within their genomic loci. Despite the emerging physiological importance of NRF family members, the repertoire of their genetic targets remains incompletely defined. Here we use RNA-sequencing-based transcriptional profiling and quantitative proteomics to delineate the overlapping and differential genetic programs effected by the three NRF transcription factors. Comparing our data to recent profiling analyses, we create consensus target gene sets regulated by NRF1, NRF2, and NRF3, genetic programs which we determine to be differentially regulated in human tissues. Together, our data provide a quantitative assessment of how NRF family members sculpt proteomes and transcriptomes, essential information for future studies evaluating the role of NRF factors in normal physiology and disease.
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| Overall design |
Comparisons of the transcriptomes of HEK293T cells expressing empty vector, FLAG-NRF1, FLAG-NRF2(T80D), or FLAG-NRF3.
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| Contributor(s) |
Ibrahim LH, Wiseman RL, Bollong MJ |
| Citation(s) |
33096892 |
| Submission date |
Oct 08, 2020 |
| Last update date |
Nov 02, 2020 |
| Contact name |
Lara Ibrahim |
| Organization name |
Scripps Research
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| Street address |
10550 N Torrey Pines Rd
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| City |
San Diego |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA668112 |
| SRA |
SRP286755 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159230_NRF_OE_Aligned_Counts.xlsx |
2.8 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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