|
| Status |
Public on Nov 01, 2020 |
| Title |
Remodeling of the m6A landscape in the heart reveals conserved post-transcriptional events underlying cardiomyocyte hypertrophy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Regulation of gene expression plays a fundamental role in cardiac stress-responses. Modification of coding transcripts by adenosine methylation (m6A) has recently emerged as a critical post-transcriptional mechanism underlying heart disease. Thousands of mammalian mRNAs are known to be m6A-modified, suggesting that remodeling of the m6A landscape may play an important role in cardiac pathophysiology. Here we found an increase in m6A content in human heart failure samples. We then adopted genome-wide analysis to define all m6A-regulated sites in human failing compared to non-failing hearts and identified targeted transcripts involved in histone modification as enriched in heart failure. Further, we compared all m6A sites regulated in human hearts with the ones occurring in isolated rat hypertrophic cardiomyocytes to define cardiomyocyte-specific m6A events conserved across species. Our results identified 38 shared transcripts targeted by m6A during stress conditions, and 11 events that are unique to unstressed cardiomyocytes. Of these, Coronin-6 (CORO6) was further evaluated for mRNA and protein abundances, demonstrating the potential impact of m6A on post-transcriptional regulation of gene expression in the heart.
|
| |
|
| Overall design |
RNA-seq and ME-RIP-seq analysis of Human heart samples undergoing cardiac hypertrophy
|
| |
|
| Contributor(s) |
Hinger SA, Wei J, He C, Accornero F |
| Citation(s) |
33188779 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL136951 |
Post-transcriptional regulation of cardiac hypertrophy |
OHIO STATE UNIVERSITY |
Federica Accornero |
|
| Submission date |
Oct 08, 2020 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Federica Accornero |
| E-mail(s) |
federica.accornero@osumc.edu
|
| Organization name |
Ohio State University Medical Center
|
| Street address |
473 W 12th Ave.
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA668129 |
| SRA |
SRP286767 |
Less...
More...