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| Status |
Public on Jul 01, 2009 |
| Title |
Mature thymic NKT and T cells |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Invariant Natural killer T (iNKT) cells are a separate lineage of T lymphocytes with innate effector functions. They express an invariant TCR specific for lipids presented by CD1d and their development and effector differentiation rely on a unique gene expression program. We asked whether this program includes microRNAs, small non-coding RNAs that regulate gene expression posttranscriptionally and play key role in the control of cellular differentiation programs. We identified a miRNA profile specific for iNKT cells, which exhibits features of activated/effector T lymphocytes.
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| Overall design |
In this experiment we compared microRNAs of NKT cells versus those of conventional T lymphocytes, both extracted from wild type mouse thymus.
To produce the populations we enriched mature thymocytes and then sorted NKT cells and conventional T cells (from one sorting we obtain one “NKT” sample and one “T” sample). We performed the experiment in triplicate; the sample NKT 1 was sorted the same day of the sample T 1, NKT 2 with T 2, NKT 3 with T 3.
We used a common reference approach for the 6 samples; as a common reference we produced RNA from total thymocytes, without the enrichment for mature cells and without the sorting; we pooled thymocytes derived from all the thymi used in the study.
The aim of the experiment was to demonstrate that NKT cells have a microRNA profile different from that of conventional T cells.
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| Contributor(s) |
Dellabona P, Casorati G |
| Citation(s) |
19625646 |
| Submission date |
May 01, 2009 |
| Last update date |
May 10, 2016 |
| Contact name |
Maya Fedeli |
| E-mail(s) |
fedeli.maya@hsr.it
|
| Phone |
00390226434735
|
| Organization name |
San Raffaele Scientific Institute
|
| Department |
DIBIT
|
| Lab |
Experimental Immunology Unit
|
| Street address |
via Olgettina 58
|
| City |
Milan |
| State/province |
MI |
| ZIP/Postal code |
20132 |
| Country |
Italy |
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| Platforms (1) |
| GPL7723 |
miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA116935 |
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