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| Status |
Public on Oct 28, 2021 |
| Title |
Human monocyte-derived macrophages polarized by GM-CSF in the presence or absence of 50 nanomolar Pemetrexed |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophage phenotypic and functional heterogeneity derives from tissue-specific transcriptional signatures shaped by the local microenvironment. GM-CSF drives the generation of human monocyte-derived macrophages with a potent pro-inflammatory activity upon stimulation. One-carbon metabolism (OCM) is a complex network of biosynthetic pathways that includes de novo biosynthesis of purines and thymidylate, amino acid metabolism, and methylation reactions. We explored the molecular impact of blocking OCM with the anti-folate pemetrexed (PMX) on the gene expression profile in GM-CSF-primed human monocyte derived macrophages.
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| Overall design |
mRNA profiles of human macrophages differentiated with GM-CSF (GM-MØ) from peripheral blood monocytes in the absence or in the presence of the antifolate pemetrexed (PMX).
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| Contributor(s) |
Romero SF, Campos RG, Kröger AP |
| Citation(s) |
34804067 |
| Submission date |
Oct 10, 2020 |
| Last update date |
Nov 30, 2021 |
| Contact name |
Amaya Rocío Puig Kröger |
| E-mail(s) |
amaya.puig@iisgm.com
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| Phone |
+34 914269268
|
| Organization name |
Hospital General Universitario Gregorio Marañón
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| Department |
Immunology
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| Lab |
Immunometabolism and Infammation
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| Street address |
Dr. Esquerdo 46
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28007 |
| Country |
Spain |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA668525 |
| SRA |
SRP286985 |
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