|
| Status |
Public on Oct 28, 2021 |
| Title |
The antifolate pemetrexed induces innate tolerance in human macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Macrophage phenotypic and functional heterogeneity derives from tissue-specific transcriptional signatures shaped by the local microenvironment. GM-CSF drives the generation of human monocyte-derived macrophages with a potent pro-inflammatory activity upon stimulation. One-carbon metabolism (OCM) is a complex network of biosynthetic pathways that includes de novo biosynthesis of purines and thymidylate, amino acid metabolism, and methylation reactions. We explored the molecular impact of blocking OCM with the anti-folate pemetrexed (PMX) on the gene expression profile in LPS-activated GM-CSF-primed human monocyte derived macrophages. The specificity of blocking OCM with the anti-folate PMX was demonstrated with folinic acid.
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| Overall design |
mRNA profiles of human monocyte-derived macrophages exposed to 10 ng/ml LPS for 3h. Monocytes were cultured for 7 days containing GM-CSF (1000 U/ml) to generate GM-CSF-polarized macrophages (GM-MØ). GM-CSF was added every two days. Pemetrexed (PMX, 50 nM), folinic acid (FA, 5 µM) or both (PMX+FA) were added once on monocytes together with GM-CSF.
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| |
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| Contributor(s) |
Romero SF, Campos RG, Kröger AP |
| Citation(s) |
34804067 |
| Submission date |
Oct 12, 2020 |
| Last update date |
Nov 30, 2021 |
| Contact name |
Amaya Rocío Puig Kröger |
| E-mail(s) |
amaya.puig@iisgm.com
|
| Phone |
+34 914269268
|
| Organization name |
Hospital General Universitario Gregorio Marañón
|
| Department |
Immunology
|
| Lab |
Immunometabolism and Infammation
|
| Street address |
Dr. Esquerdo 46
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28007 |
| Country |
Spain |
| |
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| Platforms (1) |
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| Samples (12)
|
| GSM4827531 |
LPS-treated GM-MØ macrophage donor1 rep1 |
| GSM4827532 |
LPS-treated GM-MØ macrophage donor2 rep2 |
| GSM4827533 |
LPS-treated GM-MØ macrophage donor3 rep3 |
| GSM4827534 |
LPS-treated PMX-GM-MØ macrophage donor1 rep1 |
| GSM4827535 |
LPS-treated PMX-GM-MØ macrophage donor2 rep2 |
| GSM4827536 |
LPS-treated PMX-GM-MØ macrophage donor3 rep3 |
| GSM4827537 |
LPS-treated FA-GM-MØ macrophage donor1 rep1 |
| GSM4827538 |
LPS-treated FA-GM-MØ macrophage donor2 rep2 |
| GSM4827539 |
LPS-treated FA-GM-MØ macrophage donor3 rep3 |
| GSM4827540 |
LPS-treated PMX+FA-GM-MØ macrophage donor1 rep1 |
| GSM4827541 |
LPS-treated PMX+FA-GM-MØ macrophage donor2 rep2 |
| GSM4827542 |
LPS-treated PMX+FA-GM-MØ macrophage donor3 rep3 |
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| Relations |
| BioProject |
PRJNA668719 |
| SRA |
SRP287081 |
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