 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 20, 2020 |
| Title |
Calcineurin Controls Proximodistal Blastema Polarity in Zebrafish Fin Regeneration |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Planarian flatworms regenerate their heads and tails from anterior or posterior wounds and this blastema regeneration polarity is controlled by Wnt/β-catenin signaling. It is well known that a regeneration blastema of appendages of vertebrates such as fish and amphibians grows distally. However, it remains unclear whether a regeneration blastema in vertebrate appendages can grow proximally. Here, we show that a regeneration blastema in zebrafish fins can grow proximally along the proximodistal axis by calcineurin inhibition. We used fin excavation in adult zebrafish to observe the unidirectional regeneration, from the anterior cut edge (ACE) to the posterior cut edge (PCE) of the cavity and this unidirectional regeneration polarity occurs as the PCE fails to build blastemas. Furthermore, we found that calcineurin activities in the ACE were greater than in the PCE. Calcineurin inhibition induced the PCE blastemas, and calcineurin hyperactivation suppressed fin regeneration. Collectively, these findings identify calcineurin as a molecular switch to specify the PCE blastema of the proximodistal axis and regeneration polarity in zebrafish fin.
|
| |
|
| Overall design |
Zebrafish Fin mRNA profiles of the anterior cut edge (ACE) and the posterior cut edge (PCE) at 24, 48, and 72 hpa (hours post amputation)
|
| |
|
| Contributor(s) |
Cao Z, Meng Y, Gong F, Xu C, Liu F, Fang M, Zou L, Liao X, Wang X, Luo L, Li X, Lu H |
| Citation(s) |
33376206 |
| Submission date |
Oct 19, 2020 |
| Last update date |
Jan 21, 2021 |
| Contact name |
yunlong meng |
| E-mail(s) |
yunlong_meng@126.com
|
| Phone |
15979628053
|
| Organization name |
赣南师范大学
|
| Street address |
江西省赣州市
|
| City |
赣州 |
| State/province |
江西 |
| ZIP/Postal code |
341000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA669701 |
| SRA |
SRP287505 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159560_merged_fpkm.txt.gz |
4.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...