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Status |
Public on Dec 24, 2020 |
Title |
Towards a Humanized mouse model of Pneumocystis Pneumonia |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
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Summary |
Humanized NOG-EXL (huNOG-EXL) mice control P. murina infection significantly better than control NOG-EXL mice, as evidenced by markedly decrased lung fungal burdens at both 3 and 6 weeks post-infection. Human cytokine-generating CD4+ and CD8+ T cells in the lung and serum IgM antibody, which were specifically in responses to pneumocystis antigen, were detected in huNOG-EXL mice only. For humanized NOG-EXL mouse group at 6 weeks post-infection, the whole lung RNAseq analyses were performed and the data indicated that the lower lung fungal burden is associated with higher CD209 gene expression.
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Overall design |
Groups of humanized NOG-EXL and control NOG-EXL mice were infected with Pneumocystis murina. Three and six weeks post-infection, mouse lung fungal burden, cytokine-generating cells and serum antibody levels were measured. For humanized NOG-EXL group at 6 weeks post-infection, the whole lung RNAseq was analyzed.
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Contributor(s) |
Dai G, Wanek A, Eddens T, Volden P, Song K, Kolls JK |
Citation(s) |
33491669 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 AI120033 |
Improved Therapeutics and Diagnostics for PneumocystisPneumonia |
TULANE UNIVERSITY |
JAY K KOLLS |
R35 HL139930 |
CD4_T-cell_Immunity_in_the_Lung |
TULANE UNIVERSITY |
JAY K KOLLS |
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Submission date |
Oct 19, 2020 |
Last update date |
Mar 25, 2021 |
Contact name |
Jay Kolls |
Organization name |
Tulane University
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Department |
Medicine
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Lab |
Center for Translational Research in Infection and Inflammation
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Street address |
1430 Tulane Ave
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City |
New Orleans |
State/province |
LA |
ZIP/Postal code |
70112 |
Country |
USA |
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Platforms (1) |
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Samples (4)
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Relations |
BioProject |
PRJNA669749 |
SRA |
SRP287506 |