NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE159562 Query DataSets for GSE159562
Status Public on Dec 24, 2020
Title Towards a Humanized mouse model of Pneumocystis Pneumonia
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Humanized NOG-EXL (huNOG-EXL) mice control P. murina infection significantly better than control NOG-EXL mice, as evidenced by markedly decrased lung fungal burdens at both 3 and 6 weeks post-infection. Human cytokine-generating CD4+ and CD8+ T cells in the lung and serum IgM antibody, which were specifically in responses to pneumocystis antigen, were detected in huNOG-EXL mice only. For humanized NOG-EXL mouse group at 6 weeks post-infection, the whole lung RNAseq analyses were performed and the data indicated that the lower lung fungal burden is associated with higher CD209 gene expression.
 
Overall design Groups of humanized NOG-EXL and control NOG-EXL mice were infected with Pneumocystis murina. Three and six weeks post-infection, mouse lung fungal burden, cytokine-generating cells and serum antibody levels were measured. For humanized NOG-EXL group at 6 weeks post-infection, the whole lung RNAseq was analyzed.
 
Contributor(s) Dai G, Wanek A, Eddens T, Volden P, Song K, Kolls JK
Citation(s) 33491669
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AI120033 Improved Therapeutics and Diagnostics for PneumocystisPneumonia TULANE UNIVERSITY JAY K KOLLS
R35 HL139930 CD4_T-cell_Immunity_in_the_Lung TULANE UNIVERSITY JAY K KOLLS
Submission date Oct 19, 2020
Last update date Mar 25, 2021
Contact name Jay Kolls
Organization name Tulane University
Department Medicine
Lab Center for Translational Research in Infection and Inflammation
Street address 1430 Tulane Ave
City New Orleans
State/province LA
ZIP/Postal code 70112
Country USA
 
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (4)
GSM4832516 Lung, H1_S7
GSM4832517 Lung, H3_S8
GSM4832518 Lung, H4_S9
Relations
BioProject PRJNA669749
SRA SRP287506

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE159562_abundance.fpkm.txt.gz 348.6 Kb (ftp)(http) TXT
GSE159562_abundance.htseq.txt.gz 248.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap