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| Status |
Public on Nov 18, 2021 |
| Title |
Exploring the organ specific expression pattern of CD4+iNKT1 cells of C57BL/6 and BALB/c mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
iNKT cells are generated in thymus and settle all organs and tissues in mice. We used RNA high throughput sequencing to identify genes that displayed an organ specific expression by comparing iNKT1 cells of thymus, liver, blood, and spleen origin, respectively.
We observed organ specific expression of a variety of genes that might reflect specialized functional adaptations connected to the unique micro-environments of the investigated organs.
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| Overall design |
CD4+CXCR3+iNKT1 cells were sorted out of CD3+tetramer+DAPI-B220-CD138- lymphocytes by flow cytometry (blood, liver, spleen).
For thymus, CD122 was used instead of CXCR3 and CD138 was not included as a negative marker in order to obtain CD4+iNKT1 cells.
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| Contributor(s) |
Papadogianni G, Georgiev H, Bernhardt G |
| Citation(s) |
34765746 |
| Submission date |
Oct 26, 2020 |
| Last update date |
Feb 17, 2022 |
| Contact name |
Hristo Georgiev |
| E-mail(s) |
Georgiev.Hristo@mh-hannover.de
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| Phone |
05115329731
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| Organization name |
Hannover Medical School
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| Department |
Institute of Immunology
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| Street address |
Carl Neuberg Str. 1
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| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (20)
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| GSM4861238 |
N0962: BL6 WT Blood CD4+ NKT1, 126.000 cells |
| GSM4861239 |
N0963: BL6 WT Liver digested CD4+ NKT1, 167.000 cells |
| GSM4861240 |
N0969: BL6 Liver digested NKT1 CD4+ 38.000 cells |
| GSM4861241 |
N0970: BALBc Liver on ice NKT1 CD4+ 75.000 cells |
| GSM4861242 |
N0971: BALBc Liver digested NKT1 CD4+ 14.000 cells |
| GSM4861243 |
N0972: BALBc Liver digested NKT1 CD4+ 23.000 cells |
| GSM4861244 |
N1224: BL6 liver on ice 209900 cells |
| GSM4861245 |
N1225: BL6 liver digested 33500 cells |
| GSM4861246 |
N1226: BL6 spleen 120000 cells |
| GSM4861247 |
N1227: BALBc liver on ice 208200 cells |
| GSM4861248 |
N1229: BALBc liver digested 25500 |
| GSM4861249 |
N1377: BL6 spleen CD4+ NKT1, 133000 cells |
| GSM4861250 |
N1378: BL6 blood CD4+ NKT1, 10700 cells |
| GSM4861251 |
N1379: BL6 liver on ice CD4+ NKT1, 69300 cells |
| GSM4861252 |
N1381: BL6 spleen CD4+ NKT1, 169000 cells |
| GSM4861253 |
N1382: BL6 blood CD4+ NKT1, 10700 cells |
| GSM4861254 |
N1383: BL6 liver on ice CD4+ NKT1, 87300 cells |
| GSM4861255 |
N0967: BL6 Thymus NKT1 CD4+ 121.000 cells |
| GSM4861256 |
N1376: BL6 thymus CD4+ NKT1, 83300 cells |
| GSM4861257 |
N1380: BL6 thymus CD4+ NKT1, 105000 cells |
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| Relations |
| BioProject |
PRJNA672096 |
| SRA |
SRP288620 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160144_Galaxy91-_CD4_NKT1_BL6_AllThymus_vs_AllOthers_DESeq2_result_overview_.xlsx |
19.7 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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